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- Table of Contents
Facts about Gastric inhibitory polypeptide.
Human | |
---|---|
Gene Name: | GIP |
Uniprot: | P09681 |
Entrez: | 2695 |
Belongs to: |
---|
glucagon family |
gastric inhibitory polypeptide; GIP; Glucose-dependent Insulinotropic Polypeptide; Incretin
Mass (kDA):
17.108 kDA
Human | |
---|---|
Location: | 17q21.32 |
Sequence: | 17; NC_000017.11 (48958554..48968596, complement) |
Secreted.
The Boster Microplate Reader is a versatile instrument capable of reading absorbance at 450nm. It is compatible with volumes of 0.5 ul through 1 ml of aqueous solutions. Multichannel pipettes are recommended for large samples. The reader also offers product credits for sharing results with colleagues and friends. Here are some of the most common uses of the GIP marker.
The high affinity of Boster's primary antibodies have been well-cited in the scientific literature over the past 25 years. Boster antibodies are used by many researchers and have been validated by Western Blotting, Immunohistochemistry, and ELISA. These antibodies are also highly cited in the scientific community, so researchers can rely on them to perform experiments in their labs.
The GIP series of products from Boster Bio are highly sensitive and reproducible ELISA kits. Boster validates its products on multiple platforms, including Western Blotting, Immunohistochemistry, and ELISA. The PicoKine(tm) ELISA kit is based on proprietary signal-enhancing blocking technology. The kit detects antigens at low concentrations (pg/ml) and features a very low background.
In a previous study, we validated the Boster Bio GIP markers using a mouse insulin ELISA. This assay measures plasma insulin, IGFBP3, and epiWAT. We also tested the serum and tissue IGF1 levels in mouse serum and ELISA. Using this method, we have established that Boster Bio GIP markers are highly sensitive and specific in a wide range of samples.
In addition to immunohistochemistry, Boster Bio GIP markers are thoroughly validates on Western Blotting, Immunohistochemical, and ELISA methods. Using ELISA, a secondary antibody binds to an antigen in the cell. The secondary antibody dilution factor is typically 1:200 to 1:50,000. Secondary antibodies that are affinity purified, fragmented, or pre-adsorbed are highly effective in immunohistochemistry and can penetrate tissues more easily. They also have a lower background, which means less non-specific signal.
When testing antibodies, it is important to check the specificity of the antibody. Many antibodies fail to detect their target protein when exposed to their primary antibody. This can be corrected by proper use of controls. Using multiple controls in an antibody as a control helps ensure that antibodies have specificity. This ensures that Boster Bio GIP markers are highly specific to the target protein.
In addition to these validations, the GIPs from Boster Bio are fully immuno-compatible. Immunohistochemistry is an excellent method for assessing proteins in biological samples, but Western Blotting is more specific. ELISA allows for the quantification of proteins with high-resolution. For Western Blotting, GIP-IHC proteins are the best proteins for assessing immune system response.
For a clinical diagnostic, a highly accurate GIP marker is essential. In vitro validations demonstrate that GIP-II is a specific marker for epidermal cells and smooth muscle cell regeneration. It also promotes the recruitment of epidermal stem cells and facilitates their migration and proliferation. Therefore, this marker is highly valuable for the diagnosis and treatment of epidermal diseases.
Boster Bio is an antibody manufacturing company founded in 1993. Their signature products include ELISA kits and antibodies. They have recently expanded into molecular biology products like PCR. Boster Bio also provides many services to its customers. For example, they provide free technical resources and 24 hour support to their customers. They also offer a wide range of products that are useful to scientists.
PMID: 2890159 by Takeda J., et al. Sequence of an intestinal cDNA encoding human gastric inhibitory polypeptide precursor.
PMID: 2739653 by Inagaki N., et al. Gastric inhibitory polypeptide: structure and chromosomal localization of the human gene.