FRS2 Antibodies

Fibroblast growth factor receptor substrate 2 (FRS2)

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase, in reaction to ligand-mediated activation of FGFR1.

Modulates indicating via SHC1 by competing for a common binding site on NTRK1. .

Gene Information

Human
Gene Name:	FRS2
Uniprot:	Q8WU20
Entrez:	10818

Family

Belongs to:
No superfamily

Alternative Names

FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1

Molecular Weight

Mass (kDA):
57.029 kDA

Genomic Context

Human
Location:	12q15
Sequence:	12; NC_000012.12 (69470371..69579793)

Tissue Specificity

Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Subcellular Localizations

Endomembrane system. Cytoplasmic, membrane-bound.

Diseases

• Neoplasms
• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Tumor Angiogenesis
• Neuroblastoma
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Tissue Adhesions
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Endocrine Gland Neoplasms
• Multiple Endocrine Neoplasia
• Nervousness

• Lung Neoplasms
• Prostatic Neoplasms
• Multiple Endocrine Neoplasia Type 2a
• Chimera Disorder
• Pheochromocytoma
• Chromosomal Translocation
• Neoplasm Metastasis

Interacting Proteins

• PTPN11

Pathways

• Cell Proliferation
• Localization
• Angiogenesis
• Cell Migration
• Cell Growth
• Cell Differentiation
• Cell Cycle
• Cell Death
• Tube Formation
• Chemotaxis
• Cell Adhesion
• Mesoderm Formation
• Ossification

• Chondrocyte Proliferation
• Sulfation
• Glycosylation
• Oocyte Maturation
• Regeneration
• Endothelial Cell Migration
• Mapk Cascade

PTMs

• Phosphorylation
• Ubiquitination

• Glycosylation
• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FRS2

Boster Bio: Best Uses Of The FRS2 Marker And High-Acquiring Primary Antibody

This article will focus on the Boster Bio Anti-FRS2 Marker & High-affinity Primary Antibody. It will also address the Cell-based ELISA system. It will help you decide which one is best suited for your research needs. Compare the benefits and disadvantages of each kit against their respective purposes. You will need to decide which one is best for your specific application.

Boster Bio: Anti-FRS2 marker

FRS2 is a phosphoprotein that is expressed in cells, and microbial species. There are two types that react with this protein. One type reacts with mouse cells and the other is specific to human cells. The flow procedures will vary depending on which FRS2 antibody is being used. Boster Bio provides high-affinity primary antibodies that have been validated by Western Blotting, Immunohistochemistry, and ELISA.

High-affinity primary antibody

When designing an antigen specific antibody, the first thing a biologist should look at is its affinity. High affinity is desirable but not always necessary. This property could make it more difficult to separate the antigen from the antibody, and therefore requires more aggressive methods for elution. Researchers will be able to develop a high-affinity primary antibodies by using the FRS2 marker.

Researchers need to understand the interaction between these molecules in order develop high-affinity prima antibodies. Molecular imaging can use antibody fragments as professional tools to study protein–protein and RNA–protein interactions. For example, researchers are creating new imaging methods to assess the efficacy of therapy. mAbs are known for having a high serum half life and low tumor penetration. Thus, smaller antibody fragments are needed to circumvent these limitations. This is called Nb labeling.

To determine the affinity a monoclonal immunoglobulin to a particular ligand it must be measured using the ratio between dissociation and affinity constant (KD), values of antibodies and proteins. The KD value is generally lower, which indicates that the monoclonal anti antibody has a greater affinity for a particular ligand. Scientists from Abcam analysed the OI-RD data at UC Davis.

The FRS2 marker makes it easy to develop high-affinity primaries antibodies with an optimized antigen binding domain. Single-domain antibodies exhibit low affinity, poor solubility and high production costs but they retain the high affinity properties of the parent Ab. Monoclonal Abs with high-affinity monoclonal antibody performance can be enhanced by using monoclonal Abs with high-affinity monoclonal antibodies.

ELISA test confirmed that the MBP-Lk–scFv antibody was able to bind to the IFN–g-antigen. Western blot analysis confirmed MBP-Lk–scFv binding to the IFN–g standard antigen. This indicates that the monoclonal immunoglobulin is highly active in binding antigens. This affinity constant is 2.6x109 L/mol.

Cell-based ELISA kit

Boster Bio FRS2 cell based antibody ELISA kit allows for fast and accurate analysis of the phosphorylation status Fra-2 proteins in cultured cells. This kit can be used for monitoring the relative levels in cell culture of Fra-2 proteins and to screen for activators and inhibitors of the target proteins. It is therefore suitable for screening a wide range of treatments.