DLGAP1 Antibodies

Disks large-associated protein 1 (DLGAP1)

Part of the postsynaptic scaffold in adrenal cells. .

Gene Information

Human
Gene Name:	DLGAP1
Uniprot:	O14490
Entrez:	9229

Family

Belongs to:
SAPAP family

Alternative Names

DAP1; DAP-1-ALPHA; DAP-1DAP-1-BETA; discs, large (Drosophila) homolog-associated protein 1; disks large-associated protein 1; DLGAP1; GKAP; GKAPMGC88156; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; SAPAP1Guanylate kinase-associated protein

Molecular Weight

Mass (kDA):
108.873 kDA

Genomic Context

Human
Location:	18p11.31
Sequence:	18; NC_000018.10 (3496032..4455441, complement)

Tissue Specificity

Expressed in brain.

Subcellular Localizations

Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic density. Cell junction, synapse.

Diseases

• Severe Myopia
• Infective Disorder
• Mood Disorders
• Schizophrenia
• Retinal Detachment
• Chromosome Inversion
• Glaucoma
• Blind Vision
• Myopia
• Psychotic Disorders
• Pneumonia
• Immunologic Deficiency Syndromes

• Mental Disorders
• Disorder Of Eye
• Obesity
• Cytomegalovirus Infections
• Hiv Infections
• Myopia, Degenerative

Interacting Proteins

• Dlg1
• DLG4
• GRB2
• NCK1

Pathways

• Cell Division
• Asymmetric Cell Division
• Reverse Transcription
• Coagulation
• Metaphase

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DLGAP1

Best Uses of the DLGAP1 Marker

You've reached the right place if Steven Boster is interesting in learning more. We have covered Steven Boster’s career, background, and interests. We hope you'll find this article informative and interesting. We've also included hyperlinks to some of Steven Boster’s publications. You will find out more about his research as well as how and why he became an immunochemist.

Histroy

DLGAP1 refers to a centrosomal genome gene that is expressed in many cell types. This marker is also known as the Discs-large gene. The gene is involved cell polarity as well as proliferation. Histroy Boster discovered many ways to use this marker in order to study cellular biology. We will be discussing the best uses for DLGAP1 in this article.

DLGAP1 is differentially expressed within white and brown fat cells. It regulates browning in white adipocytes and influences cell proliferation and apoptosis. Numerous studies have shown DLGAP1 may play an important role in browning the white fat. The DLGAP1 gene product is involved in type 2 diabetes, normal brain function, and other aspects of human biology.

Native DLGAP1 is dispersed in K562 and UT7 cells, but is not affected by mitotic cells. Metaphase plate-presenting cells do not contain DLGAP1. The DLGAP1 pattern is the same in K562, HEL, and UT7 cell lines. This finding is important for the understanding of cell physiology and cell culture. We found that the DLGAP1 genes are associated with a distinct genetic area.

Career

Discs-large associ protein 1 (DLGAP1), a gene that has three RISs, is called Discs. It is a member Discs–large, Scribble, Lethal Giant Larvae and Scribble pathways. It has been implicated in cell proliferation and polarity. It has many important functions. You will find new career opportunities with the DLGAP1 marking.

DLGAP1 colocalizes to gamma-tubulin, APC in centrosomes, and is associated cytoplasmic spots. In addition, DLGAP1 is organized in small granules and appears to have its own satellites independent of PCM1 centriolar satellites. It was previously suggested that DLGAP1 may have multiple functions in the cell.

The DLGAP1 protein is a key regulator of cell migration and invasion. DLGAP1 plays an important role in nerve cell signaling, synaptic formation, and nerve cell signaling in cancer cells. DLGAP1 interactions promote centrosome positioning of astrocytes. Metastasis can occur when cells lose their polarity or orientation.

Interests

DLGAP1 is a protein which selectively associates DLGs to SHANKs supercomplexes. It is responsible for the formation of the postsynaptic density (PSD), an important component of the human brain. In addition, DLGAP1 may be involved in the regulation of PSD organization by binding to SHANK proteins. These interactions could also be involved as a cause of schizophrenia and autism.

The DLGAP1 protein is expressed mainly within the cerebellum, olfactory bulb, and neocortex. These brain areas are important for symptoms of neuropsychiatric conditions. However, this marker was also studied in a wide range other species. It is also found on mice, rats, as well as humans. Despite its widespread use, we don't know much about the functions and uses of DLGAP proteins.

DLGAP1 is upregulated in schizophrenia as a result of a feedback mechanism. It is possible that neurons may be trying to return NMDA signalling and function to their normal levels. This could also explain why DLGAP1 expression has been found in the brains and brains of schizophrenia patients. These genetic changes could have serious consequences for the brain, particularly for those with mental disorders. And they could result in psychosis, autism, and other neurological conditions.

Research

The DLGAP1 gene encodes the protein disks large associated protein. This marker is found only in brain and is associated a gene variant that affects the GCM motif. This variant significantly affects DLGAP1 protein expression in whole blood cell. The DLGAP1 protein localizes to the postsynaptic density and interacts with a protein called PD95. DLGAP1 plays an important role in brain development and function.

Studies have shown DLGAP1 is expressed in different fat types, including brown and white. Dlgap1 is known to negatively regulate browning in white Adipocytes and influence the apoptosis of white fat cells. Studies using this marker to study DLGAP1's function in humans are needed to identify treatment options for obesity. It is still unknown what role this gene plays in brown fat cell growth.

DLGAP1 is present in eight species. The DLGAP gene family contains nine patient-associated missense mutations. Among these, DLGAP1-AS1 shows the highest homology with DLGAP2 and DLGAP3, while DLGAP4 is least similar. DLGAP1A2-AS2 is the most homologous among the four DLGAP1 proteins. It contains the 14-amino acid repeat domain as well as the dynein heavy chain domain and the GKAP heterology domain.

RNA samples were used to carry out the reverse transcription. The SSRT IV system by Invitrogen was used to prepare the cDNAs. The cDNAs were subjected for qPCR using a GeneRead QPCR SYBR Gold Master Mix (QIAGEN). With the RNA samples, miRNARTs were conducted to determine miR-154-5p. The internal control of DLGAP1–AS2 were U6. Each experiment was performed in three technical replicates. The 2-DDCt method was used to normalize Ct values.