COX4I1 Antibodies

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1)

This protein is one of those nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. .

Gene Information

Human
Gene Name:	COX4I1
Uniprot:	P13073
Entrez:	1327

Family

Belongs to:
cytochrome c oxidase IV family

Alternative Names

COX IV-1; COX4; COX4-1; COX4COXIV; COX4I2; Cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV; MGC72016

Molecular Weight

Mass (kDA):
19.577 kDA

Genomic Context

Human
Location:	16q24.1
Sequence:	16; NC_000016.10 (85799695..85807068)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Mitochondrion inner membrane; Single-pass membrane protein.

Diseases

• Hypoxia
• Carcinoma
• Malignant Neoplasms
• Neoplasms
• Anoxia
• Malabsorption Syndrome
• Liver Diseases
• Keratosis
• Cystic Fibrosis
• Exocrine Pancreatic Insufficiency
• Squamous Cell Carcinoma Of Skin
• Colonic Neoplasms

• Kidney Neoplasm
• Congenital Dyserythropoietic Anemia
• Mitochondrial Damage
• Endoplasmic Reticulum Stress
• Carcinogenesis
• Immunologic Deficiency Syndromes
• Insulin Resistance

Interacting Proteins

• MT-CO1
• SDCBP

Pathways

• Aging
• Response To Hypoxia
• Skeletal Muscle Adaptation
• Induction Of Apoptosis
• Insulin Secretion
• Aerobic Respiration
• Cellular Senescence
• Muscle Adaptation
• Senescence
• Mitochondrial Dna Replication
• Drug Resistance

• Dna Replication
• Reverse Transcription
• Cell Death
• Necrotic Cell Death
• Secretion

PTMs

• Oxidation

Research Areas

• Cancer
• Cellular Markers
• Hypoxia
• Lipid and Metabolism
• Loading Controls

• Neuroendocrinology

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/COX4I1

Boster Bio - Best Uses Of The COX4I1 Marker

This article will provide an overview of Boster Bio's COX4I1 Marker, as well as its validation and ELISA kit applications. In addition, we will discuss the history of Steven Boster and his company. Boster Bio is a company with a proven track record in research and development. Read on to find out more about Boster Bio and what makes them unique.

Boster Bio's COX4I1 Marker

The COX4I1 marker is a member of the cytochrome c oxidase IV family. The marker is present in the mitochondria and has two isoforms: COX4 and COXIV-1. This marker is most commonly used as a loading control and cross reacts with COX4I2.

Validation

COX4I1 gene expression is regulated by the NF-kB pathway. Hypoxia is a signaling state characterized by increased expression of COX4I2 and decreased levels of COX4I1. This process is also mediated by the transcription factor HIF1, which is not present in normal lung tissue. HIFs interact with COX4I2 to control the gene expression.

The COX4 gene is encoded by two isoforms, one of which is ubiquitous and the other tissue-specific. COX4I1 is expressed at a high level in the lungs, trachea, and placenta, but is expressed at a low level in the heart and other organs. However, studies on COX4I1 in patients with lung cancer have shown that it increases the risk of heart disease and cancer.

The COX4I2 isoform increases COX activity in the lung. COX4I2 activity is required for normal lung function, which requires energy. As the lung has the least mitochondrial content, it is possible that COX4I2 activity is expressed in environments characterized by increased activity constraints. In addition to COX4i1 gene expression, it is also essential for the lungs to resist pathological hypoxia.

A COX4I1 gene is a mitochondrial enzyme that functions in the respiratory chain complex. In response to the dissociation of Dynll1, COX4I1 generates ROS. Therefore, the COX4I1 marker is a reliable indicator of the presence of mitochondria. The COX4I1 gene is commonly used as a loading control. It is not possible to detect COX4I2 in healthy subjects without further study.

ELISA kit applications

ELISA kits for the detection of the COX4I1 marker use sandwich enzyme-linked immunosorbent assay technology. In this assay, an anti-Human COX4I1 antibody is pre-coated on 96-well plates with a biotin-conjugated detection antibody. Test samples and standards are added to the wells subsequently. The unbound conjugates are washed away with wash buffer. After that, HRP-Streptavidin is added to each well. The reaction is then visualized by comparing the signal intensity with a standard curve.

ELISA kits are used for a variety of purposes, ranging from monitoring and diagnosing autoimmune disease to monitoring the effects of drugs and other substances on the human body. ELISA kits have the advantage of being inexpensive and easy to use compared to other common tests. They are popular in the food industry for detecting allergens because they can detect allergen contaminants down to parts per million (ppm) levels. ELISA also has other uses, such as monitoring reproductive hormone levels and tracking the use of drugs.

The optimal coating conditions for the COX4I1 marker are determined by a series of experiments. The optimal coating conditions differ between antibodies and proteins, so it is important to select the right plate. A plate must have a minimum protein binding capacity of 400 ng/cm2, as these conditions ensure accurate results. Also, the type of signal to be detected determines the color of the plate. Colorimetric signals are produced on clear polystyrene flat bottom plates, whereas fluorescent ones need black or white opaque surface.

The COX4I1 ELISA kit was developed for in vitro quantitative measurement of COX4I1 in plasma, tissue homogenates, and serum samples. The recovery rates of COX4I1 are calculated by comparing the measured value to the expected amount of COX4I1 in the samples. The linearity of COX4I1 ELISA kit was tested through serial dilutions. The results were shown to be linear when compared to the expected concentrations.

A sandwich ELISA kit uses two monoclonal antibodies that target the COX4I1 gene. The primary mAb captures the antigen directly, while the secondary antibody provides the signal. The sandwich ELISA kit contains a known amount of recombinant protein. This enables the user to create a standard curve and interpret the signal from one sample against it.

Steven Boster's history

In 1993, Steve Boster developed his first product, an ELISA kit, for IHC, and soon became the largest antibody catalog in China. His innovative technologies allowed him to produce hundreds of primary antibodies. During the late 90s, Boster founded a company called PicoKine, which delivers high-sensitivity ELISA kits. In addition to providing ELISA kits, PicoKine uses proprietary trade secrets and proprietary technology to create products for use in medical research.

Steve Boster passed away on June 6, 2022 in Madison, WI. He was born in Joliet, IL, and spent most of his adult life in Joliet. His wife, Nina Mae Hall, died of cancer in 2009. He was survived by his two daughters, Natosha Peck and Crystal Boster, 6 Grandchildren, and 4 Brothers, David Boster, Jack Boster, and Sandra Blanton, as well as his niece, Tammy, and nephews.