CD200 Antibodies

OX-2 membrane glycoprotein (CD200)

Costimulates T-cell proliferation.

May regulate myeloid cell activity in many different tissues.

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Gene Information

Human
Gene Name:	CD200
Uniprot:	P41217
Entrez:	4345

Family

Belongs to:
No superfamily

Alternative Names

antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2

Molecular Weight

Mass (kDA):
31.264 kDA

Genomic Context

Human
Location:	3q13.2
Sequence:	3; NC_000003.12 (112332573..112362812)

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein.

Diseases

• Inflammation
• Neoplasms
• Autoimmune Reaction
• Malignant Neoplasms
• Leukemia
• Chronic Lymphocytic Leukemia
• Decreased Immunologic Activity [pe]
• Nervousness
• Lymphoid Leukemia
• Autoimmune Diseases
• Autoimmunity
• Arthritis

• Lymphoma
• Tissue Adhesions
• Influenza
• Sclerosis
• Neoplasm Metastasis
• Experimental Autoimmune Encephalomyelitis
• Inflammatory Response

Interacting Proteins

• CD200R1

Pathways

• Cytokine Production
• Immune Response
• Cell Activation
• Macrophage Activation
• Pathogenesis
• Aging
• Inflammatory Response
• Innate Immune Response
• Localization
• Secretion
• Phagocytosis
• Cell Adhesion
• T Cell Activation

• Cell Proliferation
• Cell Migration
• Regeneration
• Tissue Homeostasis
• Cytokine Secretion
• Mast Cell Degranulation
• Mast Cell Activation

PTMs

• Phosphorylation

• Glycosylation

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD200

Boster Bio: Best Uses Of The CD200 Marker

This article will go over the many primary antibodies that Boster offers. The antibodies were thoroughly validated for Western Blotting, Immunohistochemistry, and ELISA. They are available in a variety of sizes and have been extensively tested by the research community. Boster also has an extensive reward program for researchers who write the first review of their products. You could be awarded product credits and product recognition from Boster in the event that you write about their products.

Boster's primary antibody is frequently in the research community.

Numerous publications have recognized the high citation level Boster's primary antibody. Two UW faculty members were recently selected to the list of 2021 Highly Cited Researchers. This list is created by Clarivate which is a research company that recognizes researchers who have had substantial impact in their field through publishing numerous highly cited research papers in the last decade. This list includes papers that have received at minimum 5 citations.

Boster's primary antibodies are thoroughly validated on Western Blotting, Immunohistochemistry, and ELISA

To ensure the highest level of quality, Boster's main antibodies undergo rigorous validation tests on Western Blotting, Immunohistochemical, and ELISA. Additionally primary antibodies are distinguished by their ability to recognize the identical target proteins in a variety of tissues, cell types and cell lymphosates. These tests are designed to ensure that the primary antibodies' reactivity is identical to those of their secondary clones.

The WB method is quantitative and can detect very low concentrations of protein. Immunohistochemistry, on the other hand, uses intact tissue to visualize protein expression. WB is also utilized as a supplement to IHC tests since it gives more accurate results in quantitative terms. The epitope identified in a primary antibody may not be the same in both assays.

It is crucial to clearly document the validation of primary antibodies. The raw data related to the validation of antibodies should be provided in the publication that will allow peer review. The data should include specifics of the antibodies tested, such as the dilutions, incubation time and both positive and negative controls. The information should also include the details about the sample and the species for which the antibody was created.

Multiple independent methods are employed to validate primary antibodies used for Western Blotting. The method of immunohistochemistry uses an additional antigen to determine their specificity. Multiplexing antibodies is the newest innovation in Western Blot antibody validation. Furthermore, spectral fluorophores are also able to be conjugated to primary and secondary detection antibodies.

Different tissues and cell lines contain different proteins. Therefore, it is crucial to test the antibody on several samples. For instance, one could test the antibodies against different tissues and cell lines to determine which one binds to the same antigen. An online profiling of protein expression or RNA can be used to compare experimental results to the expected results.

Anti-cofilin antibodies can be detected by immunohistochemical analysis of the blots that contain different buffers for blocking. Odyssey CLx imaging has greater resolution and auto-intensity. The antibody ab233383 recognizes cofilin which is a 19-kDa protein. The antibody is available in 100ul vials.

Boster's primary antibodies are validated using WB

When it comes to antibodies and ELISA kits, Boster Bio is a great place to start. Boster Bio's products have been thoroughly tested and approved for use in ELISA and WB applications. The antibodies are available for mouse and human samples, and each purchase comes with a free secondary antibody. In addition to their primary antibodies, Boster also offers a assortment of kits, reagents, and ELISA controls.

An antibody is tested for the first time through Western blot. This confirms the antigen's molecular mass. This is beneficial when the antibody is able to recognize denatured antigens. A single band with known molecular mass indicates that the antibody is specific to its target. Multiple bands could represent several targets, post-translational modifications status or splice variants. Further studies are required to determine which antibody is more precise.

WB is a great initial test for validation. However WB isn't an accurate indicator of specificity of the antibody. Monoclonal antibodies work best when they bind to native proteins. WB results aren't able to establish the specificity of antibodies since denatured proteins also get denatured. The next step is to confirm the results using flow cytometry or IHC. If it is possible, it is advisable to perform negative controls before attempting to analyze specific protein with an antibody monoclonal.

Boster produces primary antibodies that are sensitive and efficient in WB assays in addition to WB validated WB. However, there are a few concerns regarding the validation of antibodies. Two different monoclonal 3D4 met antibodies that stained several breast cancer cases. The patterns of staining were distinct in one study. Researchers have developed a method to guarantee the most precise results.

As with other commercially available antibodies The phospho-specific versions of Boster's primary antibodies have been validated by WB. WB validates phospho-specific antibodies with greater specificity and validation. The company also offers recombinant antibodies production as well as an in vitro E.coli expression platform. Boster's products are widely employed in biochemical and clinical research. If you're searching for a new antibody do not hesitate to explore Boster's products.

Boster's primary antibody can be found in a variety of sizes

The Boster Bio Lab provides high-quality antibodies and ELISA kits for a range of applications. These products have been tested for IHC, WB, ELISA, FC, and ELISA-based assays. Boster also provides rabbit polyclonal antibody. Boster Bio offers a complimentary secondary antibody when you purchase a primary antibody. The products are used in a variety of biological applications like cell biology, neuroscience, and many more. The company's quality guarantee ensures that every product works exactly as promised.

Boster's ELISA kits and antibodies are validated for a variety of applications, including immunohistochemistry. Boster offers a variety of dilution factors as in a variety of sizes. Boster also provides ready-to-use antibodies that don't require either dilution or titration. Boster's antibodies are highly sensitive and specifically designed for research applications, as they have been tested against recombinant protein.

For the production of antibody recombinant, various types of animal hosts are employed. Apart from goats, chickens, sheep, and mice, various other animals are utilized for this purpose. However mice, rats and rabbits are also utilized. Other animals used to produce antibodies include guinea-pigs, mice or rats, as well as rabbits. For more specific information look up a Boster's primary antibody sales report.

It is vital to ensure that the quality of the reagents used in antibody research are of the highest quality. Boster's primary antibodies have been validated for the highest quality and purity. Boster also offers various sizes to meet different research requirements. For instance, it supplies antibodies for phosphospecific applications. Both types need to be confirmed prior to using them. There are a variety of formats and sizes of antibodies available.

Testing for quality of antibodies is necessary for the successful use of them in research. Additionally, they need to be confirmed for specificity and reproducibility. Validation should include subcellular localization, phosphospecificity, and translocations that are induced by treatment. Additionally, antibodies should be tested for dilution and buffers as well as negative and positive controls. The sensitivity of antibodies is dependent on the epitope that they are focusing on.