Clpb Antibodies

Caseinolytic peptidase B protein homolog (Clpb)

May function as a regulatory ATPase and be associated with secretion/protein trafficking process. .

Gene Information

Mouse
Gene Name:	Clpb
Uniprot:	Q60649
Entrez:	20480

Family

Belongs to:
ClpA/ClpB family

Alternative Names

caseinolytic peptidase B protein homolog; ClpB caseinolytic peptidase B homolog (E. coli); HSP78SKD3FLJ13152; Suppressor of potassium transport defect 3

Molecular Weight

Mass (kDA):
76.004 kDA

Genomic Context

Mouse
Location:	7|7 E2
Sequence:	7;

Tissue Specificity

Widely expressed, with highest levels in testis. Also expressed in heart, skeletal muscle and kidney.

Diseases

• Aortic Aneurysm, Abdominal
• Physiological Stress
• Pneumonia
• Intolerant Of Heat
• Pain
• Leishmaniasis, Cutaneous
• Infective Disorder
• Tularemia
• Dystonia Disorders
• Low Back Pain
• Tuberculosis
• Back Pain

• Actinomycetales Infections
• Anxiety Disorders
• Leishmaniasis
• Depressive Disorder
• Cell Invasion
• Dystonia Musculorum Deformans
• Horse Diseases

Pathways

• Virulence
• Proteolysis
• Localization
• Transport
• Immune Response
• Protein Folding
• Response To Heat
• Reverse Transcription
• Translation
• Response To Stress
• Quorum Sensing
• Photosynthesis
• Fermentation

• Protein Refolding
• Cold Acclimation
• Translational Initiation
• Biofilm Formation
• Dna Replication
• Pathogenesis
• Electron Transport

PTMs

• Phosphorylation
• Nitration

• Biotinylation
• Cleavage

• Methylation

Research Areas

• Protein Phosphatase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Clpb

Best Uses Of The CLPB Marker

Boster Bio is your best choice if you're looking for a primary antibody with high affinity. Boster Bio stocks a wide range high-quality antibodies, which are compatible with membrane staining. This article will provide an explanation of the CLPB Marker and its uses. It will also cover membrane staining and how efficient protein transfer is. If you are a beginner in the world of molecular biology, read on.

Boster provides high-affinity primary antibodies

Boster Bio developed ELISA tests and research antibodies that can be used in cancer, neurosciences, developmental biology, and other areas. These kits can detect biomarkers as low as a picogram. Boster Bio products are all made in-house, to ensure the highest quality. Boster antibodies will enhance your research whether you are using them for IHC and FC. Learn more about Boster antibody benefits.

Another company offering high-affinity primary antibodies is Rockland Immunochemicals, Inc. Located just west of Philadelphia, Pennsylvania, USA, Rockland produces high-affinity antibodies. These antibodies are suitable for Western blotting and ELISA as well as high-content screening applications. They also offer contract services. Boster Bio has a large selection of products that can be sourced at a reasonable price.

Boster Bio is a global supplier for high-affinity prima antibodies. Boster Bio has over 35,000 monoclonal or polyclonal antibodies as well as peptides in its portfolio. They provide researchers with high-affinity reagents that can be customized. NovoPro Bioscience also manufactures custom antibodies, peptides and other reagents to meet specific research needs. Drs. Paul Greengard, Michael Browning and Dr.

Single-domain antibody are camelid-specific proteins without a light chain. The heavy-chain variable domain is where the antigen-binding area is located. VHHs can be expressed in a simple, thermostable and easy way, but their use for small molecules detection has been limited. VHHs with low affinity have not been widely used for small-molecule detection. Most VHHs are used to detect antibodies with low affinity.

Single-cell screening has the capability to isolate high-affinity antibodies in weeks rather than years. It involves sourcing viable cellular material from patients or immunized pets. Next, single-cell sorting at high throughput is used to isolate VH/VL antibodies genes. The antibody candidate is then screened for expression and quality. It is a rapid method to identify antibodies against specific antigens and targets.

Many applications can be made of polyclonal primary antibodies. For example, researchers can analyze the response of different primary antibodies to an eluent. Polyclonal primary antibodies are highly specific because they are derived primarily from host animals. These antibodies are able recognize a wide range antigens. They can also be purified, measured, and quantified. There is one key advantage to polyclonal primary antibodies: they are easy to manufacture and measure.

Explains protein transfer efficiency by membrane staining

MemCode Reversible Protein Staining is a method of determining membrane protein transfer efficiency. It offers many advantages over Ponceau S, and is perfect for downstream applications. MemCode staining can be reversed, unlike Ponceau S. This is especially useful for blot reuse. Blue bands of protein are visible against a background that is white.

Total protein staining confirms there are proteins on a membrane and shows that the protein transfer was successful. The membrane must be stained with total protein before blocking reagent to determine the efficiency of protein transfers. The blocking agent is composed of a protein that hinders the total protein stained from binding to the membrane's protein. However, blocking substances may contain protein. This makes them less useful to electrophoretic transfer.

A significant amount of protein remains in the gel even after protein transfer. Many factors affect the transfer efficiency, including protein size and abundance, charge, hydrophobicity, and charge. Transfer of proteins with a high charge can be difficult if they are too loaded. Both methods work well for some protein transfer, but not all. The staining dye shows some protein on a membrane. It also provides an indication of how well proteins transfer between membranes.

Another way to determine transfer efficiency is to examine fluorescence patterns of recipient cells. Using mCherry as a probe, Wei et al. (2011) determined that OMmCherry was the only protein that transferred significantly between two cells. However, their study also showed that multiple cells can be involved in transfer, which may involve the alignment of cell-cells during membrane staining.

Tris-acetate gels are more effective for the transfer of higher molecular mass proteins. This can easily be improved by increasing the alcohol level of the buffer or adding SDS. Large proteins can also be transferred more efficiently with the ethanol-based gel. However, the BisTris gel can't transfer large proteins. It is important for you to know that different gels have differing transfer efficiencies. Some gels are more effective than others.

Molecular imaging provides additional information about the biology and kinetics involved in OM-mediated protein transfers. The interaction of lipids with proteins between adjacent cells promotes the transmission of proteins and/or lipids. This does not always happen. Transient OM tube formation, which is continuous extension of the OM of a single cell, is another sign that OM fusion has occurred.