CLK2 Antibodies

Dual specificity protein kinase CLK2 (CLK2)

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex.

May be a part of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can lead to redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes through its phosphorylation.

Gene Information

Human
Gene Name:	CLK2
Uniprot:	P49760
Entrez:	1196

Family

Belongs to:
protein kinase superfamily

Alternative Names

CDC-like kinase 2CLK kinase; clk2; dual specificity protein kinase CLK2; EC 2.7.12.1; hCLK2; MGC61500

Molecular Weight

Mass (kDA):
60.09 kDA

Genomic Context

Human
Location:	1q22
Sequence:	1; NC_000001.11 (155262868..155273504, complement)

Tissue Specificity

Endothelial cells.

Subcellular Localizations

Nucleus.; [Isoform 1]: Nucleus. Nucleus speckle. Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.; [Isoform 2]: Nucleus speckle. Co-localizes with serine- and arginine-rich (SR) proteins in the nuclear speckles.

Diseases

• Acute Erythroblastic Leukemia
• Ataxia
• Dementia
• Insulin Resistance
• Malignant Neoplasms
• Neoplasms
• Nervousness
• Pancreatic Neoplasm
• Lymphoma
• Cytogenetic Abnormality
• Dysequilibrium Syndrome
• Trematode Infections

• Herpes Simplex Infections
• Primary Neoplasm
• Nerve Degeneration
• Postmortem Changes
• Atrophy
• Diabetes Mellitus

Interacting Proteins

• CLK3
• CPSF7
• ECE1
• KLHL2
• LNX1

• LUZP4
• MRPL4
• PRPF38A
• RBM39
• RSRP1

• SDCBP
• SNIP1
• SNRNP70
• SRPK2
• SRRM1

• TRIM27
• YTHDC1
• ZNF263
• ZNF394
• ZNF398

• ZNF473
• ZRSR2

Pathways

• Gluconeogenesis
• Localization
• Hyperphosphorylation
• Insulin Secretion
• Brain Development
• Aging
• Dna Replication
• Cell Death
• Cellular Localization
• Cell Cycle

• Dna Replication Checkpoint
• Cell Differentiation
• Cell Growth
• Rna Processing
• Secretion

PTMs

• Phosphorylation

• Glycosylation

• Dephosphorylation

Research Areas

• Protein Kinase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CLK2

Boster Bio - Best Uses Of The CLK2 Marker In Biological Asses

In this article, we will cover the best uses of the CLK2 Marker in biological assays, including methods for immunocytochemical localization of ACHN cells. We will also cover the benefits of using Boster Bio's primary antibodies. To learn more, check out the articles below. These articles are applicable to scientists worldwide, so you can use them as needed. In addition, you can get a free trial of the CLK2 Marker by visiting Boster Bio's website.

Benefits of using the CLK2 Marker in biological assays

Using the CLK2 Marker in biological asses can enhance the performance of assays that characterize programmed cell death, measure cellular activity, and assess the response to the microenvironment. The CLK2 Marker is a versatile tool that allows scientists to study drug efficacy, toxicity, and immunogenicity. The CLK2 Marker has been used to identify and validate bioanalytes for over 20 years.

The CLK2 protein level varies across cancer cell lines and is regulated by ubiquitination. It is expressed relatively high in MDA-MB-468 cells and demonstrates a high correlation with sensitivity to chemotherapy. It is also known that CLK2 phosphorylates SR proteins and may influence alternative splicing. RNA-Seq data are also available in the Gene Expression Omnibus, with accession code GSE101540.

Increasing the responsiveness of assays can enhance the development and validation of biopharmaceutical products. Cell-based assays are a vital component of the control of biopharmaceutical quality. BioAgilytix's premier GMP lab supports all of your bioanalytical requirements, including lead optimization and mechanism-of-action studies. For more information, contact BioAgilytix's experts.

The CLK2 Kinase Enzyme System is available with or without ADP-Glo(tm) Kinase Assay reagents. They offer a convenient and flexible method for profiling kinase activity. In addition, these kits are designed for the highest-quality data. The ADP-Glo(tm) Kinase Systems are manufactured by SignalChem and are available for purchase in bulk quantities.

The CLK2 Marker is a highly sensitive tool for cancer research. It is an important indicator of MYC, an oncogene with multiple targets. It has been demonstrated that T-025 inhibits MYC-dependent cell growth. Its efficacy has been demonstrated in several solid tumor cell lines. Its effect was strongest in COLO320HSR colorectal cancer cells, while its effects were reduced in 786-O kidney cancer cells.

The CLK2 gene is amplified in a high proportion of breast tumours. The loss of CLK2 inhibits the growth of cancer cells by affecting the expression of mesenchymal splice variants of ENAH, an actin cytoskeleton protein. Moreover, inhibitors of CLK2 were shown to have significant antitumour effects. This suggests that targeting CLK2 and MYC in breast tumours may provide beneficial results in triple-negative breast cancer.

Methods for determining immunocytochemical localization of ACHN cells

In the present study, we have used anchorage-independent and anchorage-dependent culture conditions to determine the immunocytochemical localization of ACHN (adhesion-associated chondrocyte nuclei) cells. These cells form spheres, exhibiting similar properties to CSCs, but with increased tumorigenicity and invasiveness. Furthermore, we observed that SDCs expressed markers for stemness and EMT, as well as ABC transporter genes.

These results are consistent with previous studies. Cell invasion is significantly higher in SDCs compared to PCs. To test this hypothesis, we first chose an ACHN cell line and diluted it. Then, we incubated the cells for 1 hour in Calcein-AM and measured fluorescence. We then plotted the results using a trend line to compare the SDCs with PCs.

Primary antibodies from Boster Bio

A primary antibody is an antibody that reacts to a specific antigen. A Boster Bio primary antibody can be used for different types of experiments, including immunohistochemistry (IHC), western blotting, and ICC/IF. Each Boster Bio antibody is validated by multiple platforms and known positive and negative samples. The company uses highly sensitive ELISA kits to analyze the immune response. Its products have been validated for a wide range of applications, including IHC, Western blotting, and IP.

The primary antibody used by researchers is made from the immune system of the host. It is characterized by a region called the complementarity determining region. This topic is complex, and Wikipedia has an explanation. Primary antibodies are produced by immunizing the host animal with an antigen and then extracting antibodies from the host's eggs or sera. Boster also offers antibodies conjugated to multiple targets, making them a convenient choice for research and development.

In addition to this, these antibodies can be used to produce secondary antibodies. These antibodies are conjugated to other molecules, so that they can react with different antigens. Therefore, they are ideal for a wide variety of experiments. They are used in proteomics, histopathology, and Western blotting, which rely on the ability of antibodies to bind antigens. Also available are enzyme-mediated immunoassays (ELISA) and flow cytometry, which measure the amount of a certain cell type.