CILP Antibodies

Cartilage intermediate layer protein 1 (CILP)

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions.

Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1.

Gene Information

Human
Gene Name:	CILP
Uniprot:	O75339
Entrez:	8483

Family

Belongs to:
No superfamily

Alternative Names

cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; cartilage intermediate layer protein 1; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; Cartilage intermediate-layer protein; CILP; CILP1; CILP-1; HsT18872

Molecular Weight

Mass (kDA):
132.565 kDA

Genomic Context

Human
Location:	15q22.31
Sequence:	15; NC_000015.10 (65194760..65213142, complement)

Tissue Specificity

Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in hip articular cartilage. Overexpressed in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease (CPPD). Expression in intervertebral disk tissue from individuals with lumbar disk disease increases as disk degeneration progresses.

Subcellular Localizations

Secreted, extracellular space, extracellular matrix.

Diseases

• Degenerative Polyarthritis
• Intervertebral Disc Degeneration
• Calcium Pyrophosphate Deposition Disease
• Lumbar Disc Disease
• Osteoarthritis, Knee
• Arthritis
• Intervertebral Disk Displacement
• Rheumatoid Arthritis
• Arthropathy
• Lumbar Disc Degenerative Disorder
• Abnormal Degeneration
• Penile Lesion

• Autoimmunity
• Cholangiocarcinoma
• Spinal Diseases
• Arthritis, Reactive
• Brain Ischemia
• Tendon Calcification
• Coronary Artery Disease

Pathways

• Aging
• Immune Response
• Pathogenesis
• Localization
• Secretion
• Transport
• Translation
• Cholesterol Esterification

• Extracellular Transport
• Tropism

PTMs

• Glycosylation
• Phosphorylation

• Cleavage
• Citrullination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CILP

Best Uses Of The CILP Marker And RBFOX3 Antibodies

In this article, we'll discuss the CILP Marker (RBFOX3 antibodies) and Steven Boster’s historical background. We will also discuss the importance and use of the CILP Marker for biological assays. We'll also discuss why the CILP Marker can be useful for scientists and how they can make use of it to their advantage. This article was written to help scientists get the most accurate lab results.

CILP Marker

The CILP marker is a versatile tool. You can use it to create labels or color code clippings. It can be used to organize and synchronize work, and it can also be used for sorting and filtering clips. It is easier to find and retrieve markers from different parts of a videoclip by placing them on them. You can also use markers to label tasks and reduce redaction.

Double-clicking a marker once you have installed the software will bring up the dialog box. This is where you can name the markers. Then, you can add comments and set a duration for each marker, which is useful for color correction or adjusting audio levels. You can also see all of your markers, so you can quickly find one. You can quickly move to the next or preceding marker by pressing Shift/Command/M.

RBFOX3 antibodies

RBFOX3 controls alternative splicing. Also known as NeuN (HRNBP3) and NF4 (NF4). It weighs in at 33.9kg and has been found among many animal species. It regulates the alternative splicing pre-mRNA and promotes production of mRNA types that are targeted at nonsense-mediated degradation.

Researchers can use RBFOX3 antibodies to analyze RNA expression within neurons. These antibodies recognize the peptides of neuronal RNA and detect Rbfox3 antireactivity through immunoprecipitation mass spectrometry. Furthermore, the antibodies can be used to study a variety of different proteins. It can also be applied to other cell types like mammalian cells.

RNA sequencing required the isolation of RNA from the brain using a NucleoSpin (r) miRNA kit. ND1000 spectrophotometer was used to quantify RNA. Double fluorescent westernblots were then performed after single blots had been made to confirm that the target proteins had been stained. Equal amounts were separated by SDS/PAGE gels and transferred onto Nitrocellulose membranes. The membranes received a Syn I Monoclonal Rabbit Anti-NeuN Antibody and were incubated.

RBFOX3 plays a role in neuronal differentiation. It is vital for normal brain function. RBFOX3 impairment can cause synaptic transmission to be blocked, as well as long-term and short-term plasticity to be impaired. The function of RBFOX3 plays a critical role in proper hippocampal development. Dysfunctions can also affect cognition and other brain processes.

CILP Marker in biological assays

Reporter bioassays were developed to assess the physiological and cellular status for new therapeutics and vaccines. It allows the analysis of more protein types than conventional immunofluorescence stained. This versatile technique is critical in drug development. It offers many benefits. Liwei Yang, a researcher affiliated with the Renaissance School of Medicine (and the Multiplex Biotechnology Laboratory), is the principal author of the study.

Bioassays are greatly affected by nanotechnology. It is facilitating the development of nanostructures, nanodevices and nanomaterials. Nanoparticles come with a wide range in sizes and compositions. They can exhibit a wide array of new structural, electronic, catalytic, and catalytic capabilities. Nanoparticles are considered as promising alternatives to conventional reagents.

NPs are useful for multiplexed assays. However, they are not suitable for immunoassays due to some limitations. They are not suitable for multiplexed assays because of their non-specific adsorption, instability, and inherent cross-reactivity. Below are a few examples. You can also use NPs to determine multiple variables in multiplexed Immunoassays.

Nanoparticles have been used in bioanalysis for the detection of protein and nucleic acids. These technologies are a significant step forward in many areas, including gene expression profiling and high-throughput screen. There are still many hurdles to overcome when using nanoparticles. This article will address some these issues and give an overview of current status in the field.

History of Steven Boster

You have come to the right place if Steven Boster is your interest in his history. He was an American biotechnologist, who created many products in the 1990s. By the late 1990s, the company was China's largest manufacturer of catalog antibodies. He created a platform to deliver high-sensitivity ELISA samples using proprietary trade secrets. Read on to learn more about Boster's history and present.

Steve Boster's history begins with his parents, Donald and Evelyn Meier. He was a successful sales manager for many decades and was a Concordia Hall member from Staunton, VA. His 2 daughters, Crystal Boster & Natosha Pick, 6 grandchildren, four siblings, Jack Blanton & Sandra Blanton, as well his sister Lisa Milton, are his survivors. Many nieces and nephews survived Steve.