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- Table of Contents
Facts about Ubiquitin-conjugating enzyme E2 R1.
Performs ubiquitin chain elongation building ubiquitin chains in the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions'Lys-21' or'Lys-22' using a monoubiquitin.
Human | |
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Gene Name: | CDC34 |
Uniprot: | P49427 |
Entrez: | 997 |
Belongs to: |
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ubiquitin-conjugating enzyme family |
Cdc34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; E2-CDC34; EC 6.3.2.19; UBC3; UBE2R1; UBE2R1UBCH3; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
Mass (kDA):
26.737 kDA
Human | |
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Location: | 19p13.3 |
Sequence: | 19; NC_000019.10 (531720..542088) |
Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
Boster offers high affinity primary antibodies for CDC34 Marker. These antibodies have been cited over the past 25 year in scientific literature. Boster antibodies are validated on Western Blotting, Immunohistochemistry, and ELISA protocols, making them a trusted choice for research. Here are some best uses for this antibody. We have included a biography and a list with publications that highlight the history this antibody.
The CDC34tm queries strain was constructed using the standard methods of strain construction. It includes the nourseothricin N–acetyltransferase Gene from plasmid 255. To determine if the deletions cause death, OD260/280 was performed. The CDC34tm-nat1 genotype was characterized using the methods described by Goldstein and McCusker (1999).
Cdc34tm alters the expression of many substrates by inducing mutations in the genes RPN10 (Ctk2D), and Sic1 (Sic1). This strain increases Cln2 but decreases Cln1. The protein was shown as two bands. The production of Sic1 modified protein by CDC34tm-cells is also increased. These results suggest that CDC34tm cellular lethal cells have increased Sic1 protein production.
After three days of growth at 30deg, the CDC34tm xxxDKanR gene interaction was observed. During the time of the genetic interaction, CDC34tm-nat1 cells were less sensitive to acetaldehyde, and acetaldehyde-induced cells showed a higher level of ACE2 expression. Moreover, ace2 or Swi5 expression is inversely correlated the CDC34tm -nat1 gene cluster.
The CDC34tm nat1 mutation also contains 88 gene. Rpn10, a subunit that is not ATPase of the 19S regulatory part of the proteasome is one of these genes. This mutant stabilizes Sic1 through the removal of its ATP binding domain. CDC34tm-1 mutant is also fatal if RAD23 is deleted.
The CDC34tm mutant is deficient in the degradation of SCFGrr1 substrates. It also accumulates Cln1 (and Cln2), which indicate a Cln/CDK activitiy. However, Far1 is not detected in cdc34tm-1 cells. These results indicate that Cln/Cdc28 complexes can phosphorylate both Cln1 or Sic1, indicating the mutant's partial inability to degrade SCFGrr1 substrates.
Known as "he who converts science in the lavatory," Steven Boster developed his first product in 1993. He quickly developed hundreds more primary antibodies and IHC-related items. In China, he was the most prominent catalog antibody company by late nineties. PicoKine(tm) is also a proprietary ELISA platform that he developed. This proprietary ELISA platform allows for high-sensitivity ELISA kit development.
Steve Boster, 56 years old, died on June 6, 2022. He was the son James and Evelyn Meier Boster. Boster worked as a manager in retail sales for many decades. He served in the U.S. Army. His two daughters Crystal Boster and Natosha Poeck are his survivors. There are 6 grandchildren and four of his brothers, Jack Boster Sandra Blanton (and Tammy), as well. He is survived not only by his family but also by many nieces & nephews.
Wu et al. Wu et al. The current stock of these antibodies does not seem to recognize the IKBa protein. However, the species detected on blots was far below the expected molecular weights. These results have led us to revise the manuscript.
While antibodies are invaluable tools for research, many of them are lacking the requisite affinity for a particular application. A variety of approaches have been used in order to increase affinity, including random Mutagenesis and error-prone DNAPCR. Another technique is panning in solution containing enrichment of improved binding agents and competition. CDC34 mAbs are useful in many applications. The CDC34 marker is a great candidate for this process.
CDC34 CDC34 rabbit recombinant mAbs find endogenous amounts of total CDC34. CDC34 has been implicated as a factor in the ubiquitination a number of vertebrate substrates. This includes cdc34 ligases. However, CDC34 is also a phosphoprotein in proliferating cells. Phosphorylation maps on residues 203, 222 of CDC34. Mutations at Ser-236 and Thr-233 may be used to shift CDC34 from nuclear to cytoplasm.
An ELISA method is used for the purification of mAbs. 100 ml is of the supernatant and is loaded onto a single hiTrap Protein G column. This column is attached to an HPLC Infinity System. The bound antibody is eluted over 30 column volumes in a gradient of 80% MES-HCl pH 5.5 and 20% MES-HCl pH 8.8. The eluted protein can be measured at 280nm The eluated antibodies are then tested for endotoxin.
This monoclonal antibody recognizes the same epitope as clone 12CA5 and is useful for western blotting, ELISA applications, and assays based on the universal biotin-streptavidin platform. This antibody is particularly useful for the detection HA-tagged proteins. It is easy to use in western-blotting because of its low working concentration.
The C575S mtp mAb significantly increased the CDC over wild-type hIgG1. The binding of human C1q was also significantly increased by the pre-formed Hexamer. Raji cell lines, which contain physiologically relevant levels CD5930/CD55, saw the highest increase. This result is encouraging. This means that high-affinity prima antibodies using the CDC34 marker for C1q binding are more effective.
PMID: 8248134 by Plon S.E., et al. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.
PMID: 10329681 by Gonen H., et al. Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha.