BRF1 Antibodies

AND BRF1 ELISA kits, BRF1 Proteins

Many biological assays use antibodies to detect BRF1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BRF1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BRF1 antibodies...

We validate the specificity of these antibodies to BRF1 by testing them on tissues known to express BRF1 positively and negatively. Browse below to find the BRF1 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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BRF1 Infographic by Boster Bio

All BRF1 Products

Product

Figures

Specificity

Applications

Price

Anti-TFIIIB90-1 Antibody

4 Q&As

Human, Mouse

$399
Cat # A03761-1
100μl

Anti-TF3B BRF1 Antibody

1 Q&As

Human, Mouse

ELISA, IF, IHC-P, ICC, WB

$335
Cat # A30544
100ul

TF3B Colorimetric Cell-Based ELISA

Human, Mouse

ELISA

$480
Cat # EKC1894
1 kit, containing one 96-well plate and all necessary reagents

BRF1 (NM_001519) Human Over-expression Lysate

Human

$960
Cat # LS002972
100 µg

BRF1 Overview

Facts about Transcription factor IIIB 90 kDa subunit.

BRF1 3D structure. Source: alphafold

Transcription factor IIIB 90 kDa subunit (BRF1)

General activator of RNA polymerase which uses different TFIIIB complexes at distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA.

Isoform 2 is necessary for transcription of the U6 promoter. .

Gene Information

Human
Gene Name:	BRF1
Uniprot:	Q92994
Entrez:	2972

Family

Belongs to:

TFIIB family

Alternative Names

B-related factor 1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factorIIIB (S. cerevisiae); BRF-1; BRFFLJ42674; general transcription factor IIIB, 90kD subunit; GTF3BTAFIII90; hBRFMGC105048; hTFIIIB90; TAF3B2; TAF3CB - related factor 1; TATA box binding protein (TBP)-associated factor 3C; TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3Bsubunit 2; TATA box binding protein (TBP)-associated factor, RNA polymerase III, subunit 2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2; TBP - associated factor, RNA polymerase III

Molecular Weight

Mass (kDA):
73.84 kDA

Genomic Context


Human

Location:	14q32.33
Sequence:	14; NC_000014.9 (105209286..105315577, complement)

Subcellular Localizations

Nucleus.

Diseases

Neoplasms
Malignant Neoplasms
Retinoblastoma
Cell Transformation, Neoplastic
Metaplastic Cell Transformation
Carcinoma
Malignant Neoplasm Of Breast
Mammary Neoplasms
Malignant Tumor Of Cervix
Physiological Stress
Hypertrophy
Colorectal Neoplasms
Growth Arrest

Uterine Cervical Neoplasm
Colorectal Cancer
Burkitt Lymphoma
Neoplasm Metastasis
Embryonal Carcinoma
Malignant Tumor Of Colon
Hypoxia

Pathways

Translation
Cell Proliferation
Localization
Cell Growth
Cell Cycle
Hyperphosphorylation
Cellular Senescence
Regulation Of Gene Expression
Snrna Transcription
Cell Differentiation
Trna Transcription
Mitosis
Senescence

Rna Processing
Rna Interference
Transport
Stem Cell Differentiation
Cellular Homeostasis
Aging
Proteolysis

PTMs

Phosphorylation
Dephosphorylation

Cleavage
Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BRF1

References

PMID: 7624363 by Wang Z., et al. Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high- mobility-group protein 2-related domains.

PMID: 8943358 by Mital R., et al. RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB.