Atlastin-1 Antibodies

Atlastin-1 (ATL1)

GTPase tethering membranes through formation of trans- homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis (PubMed:27619977).

May also regulate Golgi biogenesis. May regulate axonal development.

Gene Information

Human
Gene Name:	ATL1
Uniprot:	Q8WXF7
Entrez:	51062

Family

Belongs to:
TRAFAC class dynamin-like GTPase superfamily

Alternative Names

AD-FSP; atlastin GTPase 1; atlastin1; atlastin-1; Brain-specific GTP-binding protein; EC 3.6.5.-; FSP1; GBP3; GBP-3; GTP-binding protein 3; Guanine nucleotide-binding protein 3; guanylate-binding protein 3; Spastic paraplegia 3 protein A; spastic paraplegia 3A (autosomal dominant); SPG3; SPG3AhGBP3

Molecular Weight

Mass (kDA):
63.544 kDA

Genomic Context

Human
Location:	14q22.1
Sequence:	14; NC_000014.9 (50533082..50633068)

Tissue Specificity

Expressed predominantly in the adult and fetal central nervous system. Measurable expression in all tissues examined, although expression in adult brain is at least 50-fold higher than in other tissues. Detected predominantly in pyramidal neurons in the cerebral cortex and the hippocampus of the brain. Expressed in upper and lower motor neurons (at protein level).

Subcellular Localizations

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell projection, axon. Localizes to endoplasmic reticulum tubular network (PubMed:27619977).

Diseases

• Spastic Paraplegia, Hereditary
• Paraplegia
• Spastic Paraplegia
• Muscle Spasticity
• Peroneal Muscular Atrophy With Pyramidal Features, Autosomal Dominant
• Weakness
• Neurodegenerative Disorders
• Fragile X Syndrome
• Paraparesis, Spastic
• Nervous System Disorder
• Spastic Paraplegia 10, Autosomal Dominant

• Cerebral Palsy
• Peripheral Neuropathy
• Weakness Of Limb
• De Novo Mutation
• Spastic Diplegia Cerebral Palsy

Interacting Proteins

• Reep5
• SPAST

Pathways

• Membrane Fusion
• Pathogenesis
• Dna Repair
• Secretory Pathway
• Membrane Tubulation
• Dna Alkylation
• Sex Determination
• Localization
• Translation
• Transport
• Cell Proliferation
• Vesicle Transport
• Fertilization

• B Cell Proliferation
• Membrane Biogenesis
• Cellular Localization
• Dna Modification
• Wound Healing
• Innervation
• Transdifferentiation

PTMs

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATL1

Atlastin-1 Antibodies - Best Uses of the ATL1 Marker

Atlastin-1, a protein, can be found throughout many tissues, including the brain and heart. This article will provide a review of the best Atlastin-1 antibodies and their uses in biological tests. In addition, we will discuss ELISA assays and the various applications for Atlastin-1. These are some helpful tips to help Atlastin-1 in biological analyses.

Atlastin-1

Atlastin-1 marks a cytoplasmic cell membrane protein that is found in eukaryotic cells' ER. It is an important component the ER–Golgi compound, which plays a part in cell-to–cell communications. Atlastin-1 plays a role in the formation vesicles within the ER. Atlastin-1 may play a role in the formation of vesicles because it is found in the ER.

This marker is able to identify human atlastin. This marker is essential for homotypic fusion. It exhibits high homo-oligomerization ability and self-association. Atlastin-HA and Myc were co-immunoprecipitated to determine whether they form homo-oligomeric complexes. Atlastin-HA was also used to study its role in ER fusion of HeLa cells.

Atlastin's involvement in the fusion ER membranes is another interesting feature. Atlastin-Myc can result in fusion when the Atlastin protein is located in the cytoplasm. It is thought that the Atlastin/Myc fusion causes excessive membrane fusion within ER cells. It also may be involved in the biogenesis of neurons and contribute to the understanding of hereditary spastic paraplegia.

Atlastin-1 antibody

Boster Bio Atlastin-1 antibody reacts with proteins from many species. These antibodies can react with Atlastin-1 in many different samples and are monoclonal, polyclonal, or polyclonal. Boster Bio uses rabbits and mice to develop Atlastin-1 antibodies. These antibodies may play a regulatory function in Golgi Biogenesis or Axonal Development. For these applications, Boster Atlastin-1 antibodies are an excellent choice.

A01994-1 refers to the anti-SPG3A/ATL1 antibody. It was used for the detection of SPG3A/ATL1 in cervical cancer tissues. The protein was electrophoresed in buffer of EDTA at pH 8.0. The sample was then transferred to sample wells under reduced conditions. The protein was then transferred to the nitrocellulose membrane by a gradient-free process for between fifty and ninety minutes. After this, the membrane was blocked in 5% non-fat milk/TBS for 1.5 hours at RT.

Atlastin-1 Assays

Boster Bio Atlastin-1's assay system uses antibodies to atlastin-1 which specifically react to this protein. Atlastin-1 is found in tissues of many animals including mice and rabbits. Boster Bio uses these two species for Atlastin-1 development. The antibodies can regulate processes, such as Golgi genesis and axonal formation. The Atlastin-1 assay was developed in mice and rabbits. It is reliable and accurate.

Atlastin (gene encoded protein) is a type of gene. It is a transmembrane GTPase and a member of the atlastin protein family. The protein can form a homotetramer and interacts with spastin. Using these assays, researchers can study atlastin and its related proteins. Atlastin is associated to hereditary spastic parplegia type 3A.

Atlastin proteins are highly conserved in the dynamin family. They are involved in the formation of vesicles that are used for secretory or endocytotic processes. In addition, atlastin proteins are needed for tubular ER morphology and function. Atlastin-dependent ER fusion requires the GTP Hydrolysis and Dimerization of Atlastin Proteins. This complex fusion helps regulate the secretory processes in cells.

Boster Bio Atlastin-1 uses a high-sensitivity ELISA platform that has picogram-level sensitivity. The ELISA kits can be validated for a variety of samples. On request, images and validation procedures can be obtained. Supervision, a polymer-based secondary antigen, saves you 30 minutes in IHC. Both Picoband and Supervision are powered by a unique platform with insights into immunogen design and support from BeNeLux distributor Sanbio.

Atlastin-1 antibodies in biological tests

Boster Bio offers Atlastin-1 antibodies as monoclonal (or polyclonal) antibodies that react to Atlastin-1 in many animal species. Atlastin-1, a GTPase as well as a transmembrane-protein, regulates axonal and Golgi development. Boster Bio validates its antibodies by comparing them to previously identified positive and/or negative samples.

Boster Atlastin-1 anti-body is intended for ELISA application. It can be augmented up to the picogram scale. This antibody was tested against 250 tissues. The antibodies are produced with high affinity and are quantitatively tested against known quantities of recombinant recombinant proteins. These antibodies can be ordered through tebu-bio, a distributor of Boster Biologicals products.