Research Spotlight - July 2023

We're delighted to bring you another issue of our Research Spotlight newsletter, showcasing the most recent papers our customers have published. These studies widen biological understanding and pave pathways for innovative therapies.

This month, we delve into a broad spectrum of biomedical research studies. We highlight research examining the intricate roles of the PD-L1/PD-1 pathway in neuroscience and the use of 3D bioprinting for biomimetic hydrogel scaffolds. Scientists demonstrate advancements in neurovascular tissue modeling, spinal cord repair, and tendon regeneration.

We also cover pioneering strategies for disease treatment, including innovative approaches to alleviate ulcerative colitis, mitigate pulmonary fibrosis, and a potential immunotherapeutic avenue for glioma.

Scroll below for digests of these exciting discoveries!

PD-L1/PD-1 Checkpoint Pathway Regulates Hippocampal Neuronal Excitability and Learning and Memory Behavior

Authors: Zhao, J., Bang, S., Furutani, K., McGinnis, A., Jiang, C., Roberts, A…
Journal: Neuron

Programmed death protein 1 (PD-1) and its ligand PD-L1 constitute an immune checkpoint pathway. We report that neuronal PD-1 signaling regulates learning/memory in health and disease. Mice lacking PD-1 (encoded by Pdcd1) exhibit enhanced long-term potentiation (LTP) and memory. Intraventricular administration of anti-mouse PD-1 monoclonal...

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Cited Boster Product(s): Mouse PD-L1/B7-H1 ELISA Kit PicoKine® (EK1450)

A PITX2–HTR1B Pathway Regulates the Asymmetric Development of Female Gonads in Chickens

Authors: Peng, Z., Man, Q., Meng, L., Wang, S., Cai, H., Zhang, C., Li, X…
Journal: PNAS Nexus

All female vertebrates develop a pair of ovaries except for birds, in which only the left gonad develops into an ovary, whereas the right gonad regresses. Previous studies found that the transcription factor Paired-Like Homeodomain 2 (PITX2), a key mediator for left/right morphogenesis in vertebrates, was also implicated in asymmetric...

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Cited Boster Product(s): Anti-DRD4 Antibody Picoband™ (A00998-2)

Generation and Characterization of a Zebrafish Model for ADGRV1-Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing Technology

Authors: Stemerdink, M., Broekman, S., Peters, T., Kremer, H., de Vrieze, E...
Journal: Cells

Worldwide, around 40,000 people progressively lose their eyesight as a consequence of retinitis pigmentosa (RP) caused by pathogenic variants in the ADGRV1 gene, for which currently no treatment options exist. A model organism that mimics the human phenotype is essential to unravel the exact pathophysiological mechanism...

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Cited Boster Product(s): Anti-Zebrafish Ush2a Antibody (DZ01481); Anti-Zebrafish Ush2c/Adgrv1 N-Terminus Antibody (DZ41032)

Disulfiram with Cu2+ Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice

Authors: Zhou, W., Zhang, H., Huang, L., Sun, C., Yue, Y., Cao, X., Jia, H…
Journal: Theranostics

Background: Disulfiram (DSF), a Food and Drug Administration (FDA)-approved drug for chronic alcohol addiction, has anti-inflammatory effects that help prevent various cancers, and Cu2+ can enhance the effects of DSF. Inflammatory bowel diseases (IBD) are characterized by chronic or recurrent relapsing gastrointestinal inflammation...

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Cited Boster Product(s): Cytoplasmic And Nuclear Protein Extraction Kit (AR0106)

3D-Printed Hydrogel Particles Containing PRP Laden With TDSCs Promote Tendon Repair in a Rat Model of Tendinopathy

Authors: Li, C., Wang, J., Yang, W., Yu, K., Hong, J., Ji, X., Yao, M., Li, S…
Journal: Journal of Nanobiotechnology

Long-term chronic inflammation after Achilles tendon injury is critical for tendinopathy. Platelet-rich plasma (PRP) injection, which is a common method for treating tendinopathy, has positive effects on tendon repair. In addition, tendon-derived stem cells (TDSCs), which are stem cells located in tendons, play a major role in maintaining...

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Cited Boster Product(s): RIPA Lysis Buffer (AR0105-100); Broad Spectrum Protease Inhibitor Cocktail (100x) (AR1182)

3D Bioprinting of Neurovascular Tissue Modeling with Collagen-Based Low-Viscosity Composites

Authors: Wang, S., Bai, L., Hu, X., Yao, S., Hao, Z., Zhou, J., Li, X., Lu, H…
Journal: Advanced Healthcare Materials

In vitro neurovascular unit (NVU) models are valuable for investigating brain functions and developing drugs. However, it remains challenging to recapitulate the native architectural features and ultra-soft extracellular matrix (ECM) properties of the natural NVU. Cell-laden bioprinting is promising to prepare complex living tissues...

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Cited Boster Product(s): DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) For Nucleic Acid Staining (AR1176)

Macrophage-Based Delivery of Anti-Fibrotic Proteins Alleviates Bleomycin-Induced Pulmonary Fibrosis in Mice

Authors: Liu, H., Yang, C., Gao, Y., Zhang, X., Wang, M., Yu, X., Wang, W…
Journal: Bioengineering & Translational Medicine

Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease characterized by chronic, progressive, and fibrotic lung injury. Although remarkable progress has been made toward understanding the pathogenesis of PF, finding more effective treatments for this fatal disease remains a challenge. In this study, we describe...

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Cited Boster Product(s): Mouse PDGF-AB ELISA Kit PicoKine® (EK0486); Mouse BMP-7 ELISA Kit PicoKine® (EK1443)

A Hyaluronic Acid/Silk Fibroin/Poly-Dopamine-Coated Biomimetic Hydrogel Scaffold With Incorporated Neurotrophin-3 for Spinal Cord Injury Repair

Authors: Sha, Q., Wang, Y., Zhu, Z., Wang, H., Qiu, H., Niu, W., Li, X., Qian, J.
Journal: Acta Biomaterialia

Bio-factor stimulation is essential for axonal regeneration in the central nervous system. Thus, persistent and efficient factor delivery in the local microenvironment is an ideal strategy for spinal cord injury repair. We developed a biomimetic hydrogel scaffold to load biofactors in situ and release them in a controlled way...

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Cited Boster Product(s): Rat Neurotrophin-3 ELISA Kit PicoKine® (EK0474)

20-Hydroxyecdysone Reprograms Amino Acid Metabolism to Support the Metamorphic Development of Helicoverpa armigera

Authors: Wang, X.P., Sun, S.P., Li, Y.X., Wang, L., Dong, D.J., Wang, J.X...
Journal: Cell Reports

Amino acid metabolism is regulated according to nutrient conditions; however, the mechanism is not fully understood. Using the holometabolous insect cotton bollworm (Helicoverpa armigera) as a model, we report that hemolymph metabolites are greatly changed from the feeding larvae to the wandering larvae and to pupae. Arginine, alpha...

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Cited Boster Product(s): Anti-ASS1 Picoband™ Antibody (A02212-1)

CDYL Knockdown Reduces Glioma Development Through an Antitumor Immune Response in the Tumor Microenvironment

Authors: Kong, J. G., Mei, Z., Zhang, Y., Xu, L.Z., Zhang, J., Wang, Y.
Journal: Cancer Letters

Gliomas are highly prevalent and aggressive brain tumors. Growing evidence shows that epigenetic changes are closely related to cancer development. Here we report the roles of Chromodomain Y-like (CDYL), an important epigenetic transcriptional corepressor in the central nervous system in glioma progression. We found that CDYL was highly...

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Cited Boster Product(s): Human CXCL14/Brak ELISA Kit PicoKine® (EK1285)