Boster Bio Life Science Blog

Antigen retrieval is a vital technique in immunohistochemistry (IHC) that enhances the visibility of antigens in formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Immunohistochemistry (IHC) is a vital technique in biomedical research and clinical diagnostics, enabling the visualization and localization of specific proteins within tissue samples. In this blog, we outline the different types of IHC staining, including direct and indirect approaches, immunofluorescence, and chromogenic techniques. We also discuss the general process of IHC staining. This overview serves as an introductory guide for understanding and implementing IHC protocols.

Types of IHC staining

Immunohistochemistry (IHC) staining is broadly categorized into several types based on the staining mechanism and the visualization methods used.

Direct Immunohistochemistry (Direct IHC): Involves the direct conjugation of the primary antibody with a detectable label (e.g., enzyme, fluorophore). This method is straightforward but may result in lower sensitivity compared to indirect methods.

Indirect Immunohistochemistry (Indirect IHC): Uses a secondary...

Immunohistochemistry (IHC) is a popular protein detection method that utilizes antibody-antigen interactions to visualize the distribution and localization of specific cellular components within cells and in their proper tissue context.

In the lab, researchers invest time and effort to optimize the sample preparation and sample staining processes of IHC. When successful, the results produce a strong and specific signal.

However, how do we know the results have been interpreted...