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Product Info Summary
| SKU: | AR1103 |
|---|---|
| Size: | 100 µl |
Product info
| Product Name | Avidin-Biotin-Peroxidase Complex for ELISA |
| SKU/Catalog Number | AR1103 |
| Pack Size | 100 µl |
| Form Supplied | Concentrated solution (100X) |
| Storage Instructions | Store at -20˚C for one year from date of receipt. Avoid repeated freeze-thaw cycles. |
| Dilution ratio | 1:100 |
| Application | Avidin-Biotin-Peroxidase Complex (ABC-HRP) reacts with HRP substrate to produce a blue color product. The complex can be used for in vitro quantitative and qualitative analysis of enzyme-linked immunoassays. |
Protocol
1.Bring the vial to room temperature (18-25°C) prior to use.
2.Centrifuge the vial prior to use.
3.Add 10 µl of Avidin-Biotin-Peroxidase Complex into 990 µl of PBS containing 2% BSA.
4.Add 100 µl of the prepared 1x Avidin-Biotin-Peroxidase Complex into each well. Cover with the plate sealer provided and incubate for 30 minutes at 37°C.
5.Wash the plate after the incubation.
6.Add 90 µl of HRP substrate to each well and incubate in the dark at 37°C.
Product Images
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For AR1103
Hello CJ!
AR1103 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of Intrathecal Injection of Prednisolone Acetate on Expression of NR2B Subunit and nNOS in Spinal Cord of Rats after Chronic Compression of Dorsal Root Ganglia
Changes in tumor necrosis factor alpha and myeloper-oxidase in mouse models of local cerebral infarction induced by photochemical method*
Traditional Chinese medicine Bao Gan Ning increase phosphorylation of CREB in liver fibrosis in vivo and in vitro
Quantitative Determination of Butocarboxim in Agricultural Products Based on Biotinylated Monoclonal Antibody
Silicon, an important exposure marker in vivo in silicosis research
Role of N‐methyl‐D‐aspartate Receptor in Hyperoxia‐Induced Lung Injury
Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates
Species: Rat
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