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Product Info Summary
| SKU: | EKC2350 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
ASK1 (Phospho-Ser83) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2350
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The ASK1 (Phospho-Ser83) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ASK1 (Phospho-Ser83) protein expression profile in cells. The kit can be used for measuring the relative amounts of ASK1 (Phospho-Ser83) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ASK1 (Phospho-Ser83).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ASK1 (Phospho-Ser83) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2350)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2350 is reactive to MAP3K5 in Human samples
Application Guarantee
EKC2350 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-ASK1 Phospho-Ser83 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-ASK1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from MDA-MB-435 cells treated with TNF-alpha
Protein Target Info & Infographic
Gene/Protein Information For MAP3K5 (Source: Uniprot.Org, NCBI)
Gene Name
MAP3K5
Full Name
Mitogen-activated protein kinase kinase kinase 5
Weight
154537 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase kinase kinase 5;2.7.11.25;Apoptosis signal-regulating kinase 1;ASK-1;MAPK/ERK kinase kinase 5;MEK kinase 5;MEKK 5;MAP3K5;ASK1, MAPKKK5, MEKK5; MAP3K5 ASK1, MAPKKK5, MEKK5 mitogen-activated protein kinase kinase kinase 5 mitogen-activated protein kinase kinase kinase 5|ASK-1|MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|MEK kinase 5|MEKK 5|apoptosis signal-regulating kinase 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MAP3K5, check out the MAP3K5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MAP3K5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ASK1 (Phospho-Ser83) Colorimetric Cell-Based ELISA Kit (EKC2350)
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