Product Info Summary
SKU: | A18139 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TTC36 Antibody Picoband®
SKU/Catalog Number
A18139
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TTC36 Antibody Picoband® catalog # A18139. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TTC36 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18139)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human TTC36 recombinant protein (Position: M1-D187). Human TTC36 shares 89.2% and 88.6% amino acid (aa) sequence identity with mouse and rat TTC36, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A18139 is reactive to TTC36 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
52588 MW
Background of TTC36
The protein encoded by this gene has three tetratricopeptide repeats and is a chaperone for heat shock protein 70. The encoded protein may function as a tumor suppressor in hepatocellular carcinoma (HCC) since it promotes apoptosis but is downregulated in HCC.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18139 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: mouse liver tissue
ICC/IF: A549 cell
FCM: 293T cell
Validation Images & Assay Conditions
![a18139 ttc36 primary antibodies wb testing 1 a18139 ttc36 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a18139-ttc36-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of TTC36 using anti-TTC36 antibody (A18139).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC36 antigen affinity purified polyclonal antibody (Catalog # A18139) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC36 at approximately 21 kDa. The expected band size for TTC36 is at 21 kDa.
![a18139 ttc36 primary antibodies if testing 2 a18139 ttc36 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a18139-ttc36-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of TTC36 using anti-TTC36 antibody (A18139) and anti-Beta Tubulin antibody (M01857-3).
TTC36 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TTC36 Antibody (A18139) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a18139 ttc36 primary antibodies fcm testing 3 a18139 ttc36 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a18139-ttc36-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of 293T cells using anti-TTC36 antibody (A18139).
Overlay histogram showing 293T cells stained with A18139 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC36 Antibody (A18139, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TTC36 (Source: Uniprot.org, NCBI)
Gene Name
TTC36
Full Name
Tetratricopeptide repeat protein 36
Weight
52588 MW
Superfamily
TTC36 family
Alternative Names
70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody TTC36 HBP21 tetratricopeptide repeat domain 36 tetratricopeptide repeat protein 36|HSP70 binding protein 21|TPR repeat protein 36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTC36, check out the TTC36 Infographic
![TTC36 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTC36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTC36 Antibody Picoband® (A18139)
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