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Product Info Summary
| SKU: | A14243 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TMEM201 Antibody Picoband®
SKU/Catalog Number
A14243
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM201 Antibody Picoband® catalog # A14243. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TMEM201 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14243)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human TMEM201 recombinant protein (Position: R38-R591). Human TMEM201 shares 83.4% amino acid (aa) sequence identity with mouse TMEM201.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14243 is reactive to TMEM201 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
72 kDa
Background of TMEM201
TMEM201 is a protein-coding gene located on chromosome 10 in humans. Its full name is Transmembrane Protein 201. This gene encodes a transmembrane protein that is predicted to play a role in cellular processes, although its exact function is not yet fully understood. Studies suggest that TMEM201 may be involved in intracellular trafficking or signaling pathways within the cell. Further research is needed to elucidate its precise biological significance and potential implications in health and disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14243 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hacat whole cell, human U251 whole cell, human Caco-2 whole cell
FCM: Caco-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TMEM201 using anti-TMEM201 antibody (A14243).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM201 antigen affinity purified polyclonal antibody (Catalog # A14243) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM201 at approximately 85 kDa. The expected band size for TMEM201 is at 72 kDa.
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Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-TMEM201 antibody (A14243).
Overlay histogram showing Caco-2 cells stained with A14243 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM201 Antibody (A14243, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TMEM201 (Source: Uniprot.org, NCBI)
Gene Name
TMEM201
Full Name
Transmembrane protein 201
Weight
72 kDa
Superfamily
TMEM201 family
Alternative Names
Transmembrane protein 201 TMEM201 Ima1, NET5, SAMP1 transmembrane protein 201 transmembrane protein 201|RP13-15M17.2|spindle-associated membrane protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM201, check out the TMEM201 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM201: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM201 Antibody Picoband® (A14243)
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