Anti-TFB2M Antibody Picoband™

Product Name

Anti-TFB2M Antibody Picoband™

View all Tfb2m Antibodies

SKU/Catalog Number

A05679-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-TFB2M Antibody Picoband™ catalog # A15780-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TFB2M Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05679-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human TFB2M recombinant protein (Position: R10-K386). Human TFB2M shares 53.2% and 54.8% amino acid (aa) sequence identity with mouse and rat TFB2M, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05679-2 is reactive to Tfb2m in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

52588 MW

Background of Tfb2m

Dimethyladenosine transferase 2; transcription factor B2, mitochondrial is an enzyme that in humans is encoded by the TFB2M gene. S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA atthe conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction withPOLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, itactivates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05679-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TFB2M using anti-TFB2M antibody (A05679-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFB2M antigen affinity purified polyclonal antibody (Catalog # A05679-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFB2M at approximately 38 kDa. The expected band size for TFB2M is at 45 kDa.

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Figure 2. Flow Cytometry analysis of K562 cells using anti-TFB2M antibody (A05679-2).
Overlay histogram showing K562 cells stained with A05679-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFB2M Antibody (A05679-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Tfb2m (Source: Uniprot.org, NCBI)

Alternative Names

dimethyladenosine transferase 2, mitochondrial; EC 2.1.1.-; FLJ22661; FLJ23182; HCV NS5A-transactivated protein 5; Hepatitis C virus NS5A-transactivated protein 5; Hkp1; h-mtTFB; h-mtTFB2; hTFB2M; Mitochondrial 12S rRNA dimethylase 2; Mitochondrial transcription factor B2; mtTFB2; NS5ATP5; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2; transcription factor B2, mitochondrial Tfb2m|Hkp, Hkp1|transcription factor B2, mitochondrial|dimethyladenosine transferase 2, mitochondrial|S-adenosylmethionine-6-N, N-adenosyl(rRNA) dimethyltransferase 2|house-keeping protein 1|mTFB2M|mitochondrial 12S rRNA dimethylase 2|mitochondrial transcription factor B2|mtTFB2

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In this infographic, you will see the following information for Tfb2m: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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