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Product Info Summary
| SKU: | A06672-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-STRN3 Antibody Picoband®
SKU/Catalog Number
A06672-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STRN3 Antibody Picoband® catalog # A06672-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-STRN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06672-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human STRN3 recombinant protein (Position: D243-H690). Human STRN3 shares 95.2% and 95.1% amino acid (aa) sequence identity with mouse and rat STRN3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06672-1 is reactive to STRN3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
94 kDa
Calculated molecular weight
87,78 kDa
Background of SG2NA
Striatin-3 is a protein that in humans is encoded by the STRN3 gene. STRN3 (Striatin 3) is a Protein Coding gene. Among its related pathways are Neurophysiological process Glutamate regulation of Dopamine D1A receptor signaling. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and calmodulin binding. An important paralog of this gene is STRN.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06672-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human K562 whole cell, human HepG2 whole cell, rat brain tissue, rat H9C2(2-1) whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
ICC/IF: HELA cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STRN3 using anti-STRN3 antibody (A06672-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat H9C2(2-1) whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRN3 antigen affinity purified polyclonal antibody (Catalog # A06672-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STRN3 at approximately 94 kDa. The expected band size for STRN3 is at 87,78 kDa.
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Figure 2. IF analysis of STRN3 using anti-STRN3 antibody (A06672-1).
STRN3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STRN3 Antibody (A06672-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HepG2 cells using anti-STRN3 antibody (A06672-1).
Overlay histogram showing HepG2 cells stained with A06672-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-STRN3 Antibody (A06672-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For STRN3 (Source: Uniprot.org, NCBI)
Gene Name
STRN3
Full Name
Striatin-3
Weight
87,78 kDa
Superfamily
WD repeat striatin family
Alternative Names
Cell cycle autoantigen SG2NA; cell cycle S/G2 nuclear autoantigen; GS2NA; S/G2 antigen; SG2NAnuclear autoantigen; striatin, calmodulin binding protein 3; striatin-3 STRN3 PPP2R6B, S/G2NA, SG2NA striatin 3 striatin-3|cell cycle S/G2 nuclear auto|cell cycle auto SG2NA|nuclear auto|protein phosphatase 2 regulatory subunit Bbeta|s/G2 |striatin, calmodulin binding protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STRN3, check out the STRN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STRN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STRN3 Antibody Picoband® (A06672-1)
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