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Product Info Summary
| SKU: | A01198-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Steroid sulfatase/STS Antibody Picoband®
View all Steroid sulfatase Antibodies
SKU/Catalog Number
A01198-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Steroid sulfatase/STS Antibody Picoband® catalog # A01198-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Steroid sulfatase/STS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01198-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Steroid sulfatase recombinant protein (Position: K297-E396).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01198-1 is reactive to STS in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
21405 MW
Background of Steroid sulfatase
Steroid sulfatase (STS), or steryl-sulfatase, formerly known as arylsulfatase C, is a sulfatase enzyme involved in the metabolism of steroids. It is encoded by the STS gene. This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01198-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human HepG2 whole cell, human PANC-1 whole cellFCM: Hela cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Steroid sulfatase using anti-Steroid sulfatase antibody (A01198-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Steroid sulfatase antigen affinity purified polyclonal antibody (Catalog # A01198-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Steroid sulfatase at approximately 65KD. The expected band size for Steroid sulfatase is at 65KD.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-Steroid sulfatase antibody (A01198-1).
Overlay histogram showing Hela cells stained with A01198-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Steroid sulfatase Antibody (A01198-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For STS (Source: Uniprot.org, NCBI)
Gene Name
STS
Full Name
Steryl-sulfatase
Weight
21405 MW
Superfamily
sulfatase family
Alternative Names
Steryl-sulfatase; Arylsulfatase C; ASC; Steroid sulfatase; Steryl-sulfate sulfohydrolase; STS; ARSC1 STS ARSC, ARSC1, ASC, ES, SSDD, XLI steroid sulfatase steryl-sulfatase|arylsulfatase C|estrone sulfatase|steroid sulfatase (microsomal), isozyme S|steryl-sulfate sulfohydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STS, check out the STS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Steroid sulfatase/STS Antibody Picoband® (A01198-1)
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Customer Q&As
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7 Customer Q&As for Anti-Steroid sulfatase/STS Antibody Picoband®
Question
Will anti-Steroid sulfatase/STS antibody A01198-1 work on feline Flow Cytometry with chorionic villus?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Our lab technicians have not tested anti-Steroid sulfatase/STS antibody A01198-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Steroid sulfatase/STS antibody A01198-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline chorionic villus in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for chorionic villus using anti-Steroid sulfatase/STS antibody A01198-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-05
Question
I was wanting to use your anti-Steroid sulfatase/STS antibody for WB for human chorionic villus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human chorionic villus identification?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can see on the product datasheet, A01198-1 anti-Steroid sulfatase/STS antibody has been validated for ELISA, Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human chorionic villus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
Is this A01198-1 anti-Steroid sulfatase/STS antibody reactive to the isotypes of STS?
J. Patel
Verified customer
Asked: 2018-12-28
Answer
The immunogen of A01198-1 anti-Steroid sulfatase/STS antibody is E. coli-derived human Steroid sulfatase recombinant protein (Position: K297-E396). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-28
Question
We are currently using anti-Steroid sulfatase/STS antibody A01198-1 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
A. Wu
Verified customer
Asked: 2018-03-02
Answer
The anti-Steroid sulfatase/STS antibody (A01198-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-02
Question
I have a question about product A01198-1, anti-Steroid sulfatase/STS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01198-1 anti-Steroid sulfatase/STS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-30
Question
See attached the WB image, lot number and protocol we used for chorionic villus using anti-Steroid sulfatase/STS antibody A01198-1. Please let me know if you require anything else.
W. Parker
Verified customer
Asked: 2014-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-23
