Anti-SNW1 Antibody (C-Term)

Product Name

Anti-SNW1 Antibody (C-Term)

View all SNW1 Antibodies

SKU/Catalog Number

M03854

Size

50 µl

Description

Boster Bio Anti-SNW1 Antibody (C-Term) (Catalog # M03854). Tested in WB, IF application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SNW1 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03854)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This SNW1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 464-497 amino acids from human SNW1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03854 is reactive to SNW1 in Human, Mouse

Applications

M03854 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

61494 Da

Background of SNW1

Involved in transcriptional regulation. Modulates TGF- beta-mediated transcription via association with SMAD proteins, MYOD1-mediated transcription via association with PABPN1, RB1- mediated transcriptional repression, and retinoid-X receptor (RXR)- and vitamin D receptor (VDR)-dependent gene transcription in a cell line-specific manner probably involving coactivators NCOA1 and GRIP1. Is involved in NOTCH1-mediated transcriptional activation. Binds to multimerized forms of Notch intracellular domain (NICD) and is proposed to recruit transcriptional coactivators such as MAML1 to form an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ to form a transcriptional activation complex by releasing SNW1 and redundant NOTCH1 NICD. Proposed to be involved in transcriptional activation by EBV EBNA2 of CBF-1/RBPJ-repressed promoters. Is recruited by HIV-1 Tat to Tat:P-TEFb:TAR RNA complexes and is involved in Tat transcription by recruitment of MYC, MEN1 and TRRAP to the HIV promoter. Functions as a splicing factor in pre-mRNA splicing. Is required in the specific splicing of CDKN1A pre-mRNA; the function probbaly involves the recruitment of U2AF2 to the mRNA. Is proposed to recruit PPIL1 to the spliceosome. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SNW1 with M03854 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).

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All lanes : Anti-SNW1 Antibody (C-Term) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: Raji whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For SNW1 (Source: Uniprot.org, NCBI)

Alternative Names

Bx42; homolog of Drosophila BX42; MGC119379; NCoA-62; Nuclear protein SkiP; Nuclear receptor coactivator NCoA-62; nuclear receptor coactivator, 62-kD; Prp45; PRPF45; SKI interacting protein; Ski-interacting protein; SKIPSKIIP; SNW domain containing 1; SNW domain-containing protein 1 SNW1 Bx42, FUN20, NCOA-62, PRPF45, Prp45, SKIIP, SKIP, SKIP1 SNW domain containing 1 SNW domain-containing protein 1|SKI interacting protein|homolog of Drosophila BX42|nuclear protein SkiP|nuclear receptor coactivator NCoA-62|nuclear receptor coactivator, 62-kD|ski-interacting protein

For more info on SNW1, check out the SNW1 Infographic

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In this infographic, you will see the following information for SNW1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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