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Product Info Summary
| SKU: | A01062 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Ski oncogene Ski Antibody
SKU/Catalog Number
A01062
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ski oncogene Ski Antibody (Catalog # A01062). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
Ski antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Ski oncogene Ski Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01062)
Host
Rabbit
Contents
Ski Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TFPI antibody is human, mouse and rat reactive. At least two isoforms of TFPI are known to exist; this antibody will detect both isoforms.
Reactive Species
A01062 is reactive to SKI in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
80005 MW
Background of SKI
TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01062 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
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Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SKI (Source: Uniprot.org, NCBI)
Gene Name
SKI
Full Name
Ski oncogene
Weight
80005 MW
Superfamily
SKI family
Alternative Names
SGS, SKV, Ski oncogene, Proto-oncogene c-Ski SKI SGS, SKV SKI proto-oncogene ski oncogene|proto-oncogene c-Ski|ski oncoprotein|v-ski avian sarcoma viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKI, check out the SKI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ski oncogene Ski Antibody (A01062)
Hello CJ!
A01062 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang H,Wang JS,Chen XG,Kang L,Lin MB.Overexpression of c-Ski promotes cell proliferation, invasion and migration of gastric cancer associated fibroblasts.Kaohsiung J Med Sci.2019 Apr;35(4):214-221.doi:10.1002/kjm2.12042.Epub 2019 Mar 21.PMID:30896889.
Species: Human
A01062 usage in article: APP:IHC, SAMPLE:GASTRIC TISSUE, DILUTION:1:100
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