Anti-SH2D3C Antibody Picoband™

Product Name

Anti-SH2D3C Antibody Picoband™

View all SH2D3C Antibodies

SKU/Catalog Number

A04497-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SH2D3C Antibody Picoband™ catalog # A04497-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SH2D3C Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04497-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human SH2D3C recombinant protein (Position: D48-H789). Human SH2D3C shares 86.1% amino acid (aa) sequence identity with mouse SH2D3C.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04497-1 is reactive to SH2D3C in Human

Applications

A04497-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

94 kDa

Calculated molecular weight

52588 MW

Background of SH2D3C

SH2 domain containing 3C, also known as SH2D3C, is a protein that in humans is encoded by the SH2D3C gene. This gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein contains a putative Src homology 2 (SH2) domain and guanine nucleotide exchange factor-like domain which allows this signaling protein to form a complex with scaffolding protein Crk-associated substrate. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SH2D3C using anti-SH2D3C antibody (A04497-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D3C antigen affinity purified polyclonal antibody (Catalog # A04497-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH2D3C at approximately 94 kDa. The expected band size for SH2D3C is at 94 kDa.

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Figure 2. Flow Cytometry analysis of HEL cells using anti-SH2D3C antibody (A04497-1).
Overlay histogram showing HEL cells stained with A04497-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH2D3C Antibody (A04497-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SH2D3C (Source: Uniprot.org, NCBI)

Alternative Names

CHAT; Novel SH2-containing protein 3; NSP3FLJ39664; PRO34088; SH2 domain containing 3C; SH2 domain-containing 3C; SH2 domain-containing protein 3C SH2D3C CHAT, NSP3, PRO34088, SHEP1 SH2 domain containing 3C SH2 domain-containing protein 3C|SH2 domain-containing Eph receptor-binding protein 1|cas/HEF1-associated signal transducer|chat-H|novel SH2-containing protein 3

For more info on SH2D3C, check out the SH2D3C Infographic

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In this infographic, you will see the following information for SH2D3C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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