Click image to see more details
Product Info Summary
| SKU: | PB10096 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SCTR Antibody Picoband®
View all Secretin R Antibodies
SKU/Catalog Number
PB10096
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SCTR Antibody Picoband® catalog # PB10096. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCTR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10096)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SCTR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10096 is reactive to SCTR in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
59 kDa
Calculated molecular weight
50207 MW
Background of Secretin R
Human secretin receptor (gene name SCTR) is a G protein-coupled receptor and belongs to the glucagon-VIP-secretin receptor family. It binds secretin which is the most potent regulator of pancreatic bicarbonate, electrolyte and volume secretion. Secretin and its receptor are suggested to be involved in pancreatic cancer and autism. The SCTR gene is mapped to chromosome 2q14.1 by fluorescence in situ hybridization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10096 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat kidney tissue, SKOV3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SCTR using anti-SCTR antibody (PB10096).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: SKOV3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCTR antigen affinity purified polyclonal antibody (Catalog # PB10096) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCTR at approximately 59 kDa. The expected band size for SCTR is at 50 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SCTR (Source: Uniprot.org, NCBI)
Gene Name
SCTR
Full Name
Secretin receptor
Weight
50207 MW
Superfamily
G-protein coupled receptor 2 family
Alternative Names
Secretin receptor;SCT-R;SCTR; SCTR SR secretin receptor secretin receptor|pancreatic secretin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCTR, check out the SCTR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCTR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCTR Antibody Picoband® (PB10096)
Hello CJ!
No publications found for PB10096
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SCTR Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SCTR Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-SCTR Antibody Picoband®
Question
Is there a BSA free version of anti-SCTR antibody PB10096 available?
B. Anderson
Verified customer
Asked: 2020-01-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SCTR antibody PB10096 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-31
Question
Would anti-SCTR antibody PB10096 work on monkey WB with pancreas?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
Our lab technicians have not tested anti-SCTR antibody PB10096 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-SCTR antibody PB10096 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-14
Question
We are currently using anti-SCTR antibody PB10096 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
The anti-SCTR antibody (PB10096) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-28
