Anti-SCFD2 Antibody Picoband™

Product Name

Anti-SCFD2 Antibody Picoband™

View all SCFD2 Antibodies

SKU/Catalog Number

A15482-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SCFD2 Antibody Picoband™ catalog # A15482-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SCFD2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15482-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SCFD2 recombinant protein (Position: K20-H585).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A15482-1 is reactive to SCFD2 in Human, Mouse

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

75 kDa

Calculated molecular weight

24145 MW

Background of SCFD2

SCFD2 (sec1 family domain containing 2), also known as STXBP1L1 (syntaxinbinding protein 1-like 1), is a 684 amino acid protein suggested to play a role in protein transport. Existing as two alternatively spliced isoforms, SCFD2 is a member of the STXBP/unc-18/SEC1 family and is encoded by a gene that maps to human chromosome 4q12. Chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington’s disease, is on chromosome 4. FGFR-3 is also encoded on chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A15482-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SCFD2 using anti-SCFD2 antibody (A15482-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCFD2 antigen affinity purified polyclonal antibody (Catalog # A15482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCFD2 at approximately 75 kDa. The expected band size for SCFD2 is at 75 kDa.

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Figure 2. IF analysis of SCFD2 using anti-SCFD2 antibody (A15482-1).
SCFD2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCFD2 Antibody (A15482-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-SCFD2 antibody (A15482-1).
Overlay histogram showing SH-SY5Y cells stained with A15482-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SCFD2 Antibody (A15482-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SCFD2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ21060; FLJ39514; sec1 family domain containing 2; STXBP1L1sec1 family domain-containing protein 2; syntaxin binding protein 1-like 1; Syntaxin-binding protein 1-like 1 SCFD2 STXBP1L1 sec1 family domain containing 2 sec1 family domain-containing protein 2|syntaxin binding protein 1-like 1

For more info on SCFD2, check out the SCFD2 Infographic

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In this infographic, you will see the following information for SCFD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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