Click image to see more details
Product Info Summary
| SKU: | A12576-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RTKN2 Antibody Picoband®
SKU/Catalog Number
A12576-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RTKN2 Antibody Picoband® catalog # A12576-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RTKN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12576-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human RTKN2 recombinant protein (Position: M1-D572). Human RTKN2 shares 71.8% amino acid (aa) sequence identity with mouse RTKN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12576-1 is reactive to RTKN2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
69 kDa
Background of Rtkn2
Rhotekin 2 is a protein in humans that is encoded by the RTKN2 gene. The RTKN2 gene encodes a protein known as Rhotekin-2, which is involved in various cellular processes, including cytoskeletal organization, cell migration, and signal transduction. RTKN2 interacts with Rho GTPases, particularly RhoA, and modulates their activity to regulate actin cytoskeleton dynamics and cell morphology. This protein plays a role in cell adhesion and migration, with implications for tissue development, wound healing, and cancer metastasis. Additionally, RTKN2 has been linked to synaptic plasticity and neuronal development in the brain. Dysregulation of RTKN2 expression or function has been associated with neurological disorders, cardiovascular diseases, and cancer progression. Elucidating the molecular mechanisms underlying RTKN2's diverse functions is crucial for understanding its physiological roles and its potential as a therapeutic target for various diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12576-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
.
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HL-60 whole cell, human Raji whole cell, human 293T whole cell, human A431 whole cell, rat lung tissue, rat brain tissue, mouse lung tissue, mouse brain tissue
FCM: Raji cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RTKN2 using anti-RTKN2 antibody (A12576-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTKN2 antigen affinity purified polyclonal antibody (Catalog # A12576-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTKN2 at approximately 69 kDa. The expected band size for RTKN2 is at 69 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of Raji cells using anti-RTKN2 antibody (A12576-1).
Overlay histogram showing Raji cells stained with A12576-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTKN2 Antibody (A12576-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RTKN2 (Source: Uniprot.org, NCBI)
Gene Name
RTKN2
Full Name
Rhotekin-2
Weight
69 kDa
Alternative Names
bA531F24.1; DKFZp686J10120; Em:AC024597.2; FLJ39352; PH domain-containing family K member 1; pleckstrin homology domain containing, family K member 1; Pleckstrin homology domain-containing family K member 1; PLEKHK1; rhotekin 2; rhotekin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RTKN2, check out the RTKN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RTKN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RTKN2 Antibody Picoband® (A12576-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RTKN2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RTKN2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
