Anti-RTKN2 Antibody Picoband™

Product Name

Anti-RTKN2 Antibody Picoband™

View all Rtkn2 Antibodies

SKU/Catalog Number

A12576-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RTKN2 Antibody Picoband™ catalog # A12576-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RTKN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12576-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human RTKN2 recombinant protein (Position: M1-D572). Human RTKN2 shares 71.8% amino acid (aa) sequence identity with mouse RTKN2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12576-1 is reactive to Rtkn2 in Human, Mouse, Rat

Applications

A12576-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

69 kDa

Background of Rtkn2

Rhotekin 2 is a protein in humans that is encoded by the RTKN2 gene. The RTKN2 gene encodes a protein known as Rhotekin-2, which is involved in various cellular processes, including cytoskeletal organization, cell migration, and signal transduction. RTKN2 interacts with Rho GTPases, particularly RhoA, and modulates their activity to regulate actin cytoskeleton dynamics and cell morphology. This protein plays a role in cell adhesion and migration, with implications for tissue development, wound healing, and cancer metastasis. Additionally, RTKN2 has been linked to synaptic plasticity and neuronal development in the brain. Dysregulation of RTKN2 expression or function has been associated with neurological disorders, cardiovascular diseases, and cancer progression. Elucidating the molecular mechanisms underlying RTKN2's diverse functions is crucial for understanding its physiological roles and its potential as a therapeutic target for various diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
. Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RTKN2 using anti-RTKN2 antibody (A12576-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTKN2 antigen affinity purified polyclonal antibody (Catalog # A12576-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTKN2 at approximately 69 kDa. The expected band size for RTKN2 is at 69 kDa.

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Figure 2. Flow Cytometry analysis of Raji cells using anti-RTKN2 antibody (A12576-1).
Overlay histogram showing Raji cells stained with A12576-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTKN2 Antibody (A12576-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Rtkn2 (Source: Uniprot.org, NCBI)

Alternative Names

bA531F24.1; DKFZp686J10120; Em:AC024597.2; FLJ39352; PH domain-containing family K member 1; pleckstrin homology domain containing, family K member 1; Pleckstrin homology domain-containing family K member 1; PLEKHK1; rhotekin 2; rhotekin-2

For more info on Rtkn2, check out the Rtkn2 Infographic

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In this infographic, you will see the following information for Rtkn2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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