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Product Info Summary
| SKU: | A00444-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ROR gamma/RORC Antibody Picoband®
SKU/Catalog Number
A00444-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ROR gamma/RORC Antibody Picoband® catalog # A00444-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ROR gamma/RORC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00444-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ROR gamma/RORC recombinant protein (Position: Q286-E511).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00444-3 is reactive to RORC in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
58.195kDa
Background of RORC
RAR-related orphan receptor gamma (RORγ) is a protein that in humans is encoded by the RORC (RAR-related orphan receptor C) gene. The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00444-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HCCT tissue, human MOLT-4 whole cell
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ROR gamma/RORC using anti-ROR gamma/RORC antibody (A00444-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR gamma/RORC antigen affinity purified polyclonal antibody (Catalog # A00444-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR gamma/RORC at approximately 58 kDa. The expected band size for ROR gamma/RORC is at 58 kDa.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-ROR gamma/RORC antibody (A00444-3).
Overlay histogram showing Hela cells stained with A00444-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR gamma/RORC Antibody (A00444-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RORC (Source: Uniprot.org, NCBI)
Gene Name
RORC
Full Name
Nuclear receptor ROR-gamma
Weight
58.195kDa
Superfamily
nuclear hormone receptor family
Alternative Names
Coagulation factor V; Activated protein C cofactor; Proaccelerin, labile factor; Coagulation factor V heavy chain; Coagulation factor V light chain; F5 RORC IMD42, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RAR related orphan receptor C nuclear receptor ROR-gamma|RAR-related orphan nuclear receptor variant 2|nuclear receptor RZR-gamma|nuclear receptor subfamily 1 group F member 3|retinoic acid-binding receptor gamma|retinoid-related orphan receptor gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RORC, check out the RORC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RORC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROR gamma/RORC Antibody Picoband® (A00444-3)
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