Anti-RNF43 Antibody Picoband®

Product Name

Anti-RNF43 Antibody Picoband®

View all RNF43 Antibodies

SKU/Catalog Number

A01694-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNF43 Antibody Picoband® catalog # A01694-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNF43 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01694-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01694-1 is reactive to RNF43 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

86 kDa

Calculated molecular weight

85.722kDa

Background of RNF43

RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01694-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml
ELISA,0.1-0.5μg/ml

Positive Control

WB: human Hela whole cell, human 293T whole cell, human COLO-320 whole cell, human SW620 whole cell, human MCF-7 whole cell

Validation Images & Assay Conditions

Gene/Protein Information For RNF43 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RING-type E3 ubiquitin transferase RNF43; RNF43 RNF43 RNF124, SSPCS, URCC ring finger protein 43 E3 ubiquitin-protein ligase RNF43|RING-type E3 ubiquitin transferase RNF43

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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