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Product Info Summary
| SKU: | M02875 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-RNF4 Antibody
SKU/Catalog Number
M02875
Size
50 µl
Description
Boster Bio Anti-RNF4 Antibody (Catalog # M02875). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RNF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02875)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2055CT191.101.249
Isotype
IgG1,k
Immunogen
This RNF4 antibody is generated from a mouse immunized with a recombinant protein from the human region of human RNF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02875 is reactive to RNF4 in Human
Reconstitution
Calculated molecular weight
21319 Da
Background of RNF4
E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02875 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-RNF4 Antibody at 1:2000 dilution
Lane 1: LNCap whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For RNF4 (Source: Uniprot.org, NCBI)
Gene Name
RNF4
Full Name
E3 ubiquitin-protein ligase RNF4
Weight
21319 Da
Alternative Names
E3 ubiquitin-protein ligase RNF4, 6.3.2.-, RING finger protein 4, Small nuclear ring finger protein, Protein SNURF, RNF4, SNURF RNF4 RES4-26, SLX5, SNURF ring finger protein 4 E3 ubiquitin-protein ligase RNF4|E3 ubiquitin ligase RNF4|RING-type E3 ubiquitin transferase RNF4|small nuclear RING finger protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF4, check out the RNF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF4 Antibody (M02875)
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No publications found for M02875
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