Anti-RNF4 Antibody

Product Name

Anti-RNF4 Antibody

View all RNF4 Antibodies

SKU/Catalog Number

M02875

Size

50 µl

Description

Boster Bio Anti-RNF4 Antibody (Catalog # M02875). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RNF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02875)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

2055CT191.101.249

Isotype

IgG1,k

Immunogen

This RNF4 antibody is generated from a mouse immunized with a recombinant protein from the human region of human RNF4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02875 is reactive to RNF4 in Human

Applications

M02875 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21319 Da

Background of RNF4

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-RNF4 Antibody at 1:2000 dilution
Lane 1: LNCap whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For RNF4 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin ligase RNF4; EC 6.3.2.-; Protein SNURF; RES4-26; ring finger protein 4SNURFSLX5; RNF4; SLX5; Small nuclear ring finger protein; SNURF RNF4 RES4-26, SLX5, SNURF ring finger protein 4 E3 ubiquitin-protein ligase RNF4|E3 ubiquitin ligase RNF4|RING-type E3 ubiquitin transferase RNF4|small nuclear RING finger protein

For more info on RNF4, check out the RNF4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RNF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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