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Product Info Summary
| SKU: | A02521-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RNase L/Rnasel Antibody Picoband®
SKU/Catalog Number
A02521-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RNase L/Rnasel Antibody Picoband® catalog # A02521-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNase L/Rnasel Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02521-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse RNase L/Rnasel recombinant protein (Position: E24-S735).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02521-1 is reactive to Rnasel in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated molecular weight
87164 MW
Background of RNase L
RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02521-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse lung tissue, mouse Raw2647 whole cell
FCM: HEPA1-6 cell, NRK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RNase L/Rnasel using anti-RNase L/Rnasel antibody (A02521-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse Raw264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNase L/Rnasel antigen affinity purified polyclonal antibody (Catalog # A02521-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNase L/Rnasel at approximately 83KD. The expected band size for RNase L/Rnasel is at 83KD.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-RNase L/Rnasel antibody (A02521-1).
Overlay histogram showing HEPA1-6 cells stained with A02521-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNase L/Rnasel Antibody (A02521-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of NRK cells using anti-RNase L/Rnasel antibody (A02521-1).
Overlay histogram showing NRK cells stained with A02521-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNase L/Rnasel Antibody (A02521-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Rnasel (Source: Uniprot.org, NCBI)
Gene Name
Rnasel
Full Name
2-5A-dependent ribonuclease
Weight
87164 MW
Superfamily
protein kinase superfamily
Alternative Names
Endothelin-converting enzyme 1;ECE-1;3.4.24.71;ECE1; RNASEL PRCA1, RNS4 ribonuclease L 2-5A-dependent ribonuclease|2,5-oligoisoadenylate synthetase-dependent|2-5A-dependent RNase|RNase L|interferon-induced 2-5A-dependent RNase|ribonuclease 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rnasel, check out the Rnasel Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rnasel: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNase L/Rnasel Antibody Picoband® (A02521-1)
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