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Product Info Summary
| SKU: | A07833-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RIT2 Antibody Picoband®
SKU/Catalog Number
A07833-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIT2 Antibody Picoband® catalog # A07833-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RIT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07833-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RIT2 recombinant protein (Position: Q119-K193).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07833-1 is reactive to RIT2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
17,21 kDa
Calculated molecular weight
49229 MW
Background of RIT2
GTP-binding protein Rit2 is a protein that in humans is encoded by the RIT2 gene. RIN belongs to the RAS (HRAS; MIM 190020) superfamily of small GTPases
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07833-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human SH-SY5Y whole cell, human SK-N-SH whole cell, human U-87MG whole cell, human U251 whole cell, rat brain tissue, mouse brain tissue
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RIT2 using anti-RIT2 antibody (A07833-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human SK-N-SH whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIT2 antigen affinity purified polyclonal antibody (Catalog # A07833-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIT2 at approximately 17,21 kDa. The expected band size for RIT2 is at 25 kDa.
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Figure 2. Flow Cytometry analysis of U20S cells using anti-RIT2 antibody (A07833-1).
Overlay histogram showing U20S cells stained with A07833-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIT2 Antibody (A07833-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RIT2 (Source: Uniprot.org, NCBI)
Gene Name
RIT2
Full Name
GTP-binding protein Rit2
Weight
49229 MW
Superfamily
small GTPase superfamily
Alternative Names
Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27 RIT2 RIBA, RIN, ROC2 Ras like without CAAX 2 GTP-binding protein Rit2|GTP-binding protein Roc2|Ric-like, expressed in neurons|ras-like protein expressed in neurons|ras-like without CAAX protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIT2, check out the RIT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIT2 Antibody Picoband® (A07833-1)
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