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Product Info Summary
| SKU: | A13755 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RHNO1 Antibody Picoband®
SKU/Catalog Number
A13755
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RHNO1 Antibody Picoband® catalog # A13755. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RHNO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13755)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RHNO1, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat RHNO1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13755 is reactive to RHNO1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
64099 MW
Background of Rhno1
Involved in cellular response to radiation; recombinational repair; and regulation of cell cycle process. Located in chromosome and nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13755 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
Positive Control
WB: human MOLT-4 whole cell, human U251 whole cell
FCM: RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RHNO1 using anti-RHNO1 antibody (A13755).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHNO1 antigen affinity purified polyclonal antibody (Catalog # A13755) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHNO1 at approximately 40 kDa. The expected band size for RHNO1 is at 27 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-RHNO1 antibody (A13755).
Overlay histogram showing RT4 cells stained with A13755 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RHNO1 Antibody (A13755, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RHNO1 (Source: Uniprot.org, NCBI)
Gene Name
RHNO1
Full Name
RAD9, HUS1, RAD1-interacting nuclear orphan protein 1
Weight
64099 MW
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHNO1, check out the RHNO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHNO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RHNO1 Antibody Picoband® (A13755)
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