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Product Info Summary
| SKU: | M01299-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Retinoid X Receptor alpha Rabbit Monoclonal Antibody
View all RXR alpha/NR2B1 Antibodies
SKU/Catalog Number
M01299-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Retinoid X Receptor alpha Rabbit Monoclonal Antibody catalog # M01299-2. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Retinoid X Receptor alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01299-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20R23
Isotype
IgG
Immunogen
A synthesized peptide derived from human Retinoid X Receptor alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01299-2 is reactive to RXRA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
51 kDa
Calculated molecular weight
51 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01299-2 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Retinoid X Receptor Alpha using anti-Retinoid X Receptor Alpha antibody (M01299-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Retinoid X Receptor Alpha antigen affinity purified monoclonal antibody (Catalog # M01299-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Retinoid X Receptor Alpha at approximately 51 kDa. The expected band size for Retinoid X Receptor Alpha is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RXRA (Source: Uniprot.org, NCBI)
Gene Name
RXRA
Full Name
Retinoic acid receptor RXR-alpha
Weight
51 kDa
Superfamily
nuclear hormone receptor family
Alternative Names
Paired box protein Pax-2;PAX2; RXRA NR2B1 retinoid X receptor alpha retinoic acid receptor RXR-alpha|nuclear receptor subfamily 2 group B member 1|retinoid X nuclear receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RXRA, check out the RXRA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RXRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Retinoid X Receptor alpha Rabbit Monoclonal Antibody (M01299-2)
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