Anti-Ras-related protein Rab-21 RAB21 Antibody

Product Name

Anti-Ras-related protein Rab-21 RAB21 Antibody

View all RAB21 Antibodies

SKU/Catalog Number

A05683

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ras-related protein Rab-21 RAB21 Antibody (Catalog # A05683). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RAB21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Ras-related protein Rab-21 RAB21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05683)

Host

Rabbit

Contents

RAB21 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21. The immunogen is located within the last 50 amino acids of RAB21.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody is predicted to not cross-react with other Ras-related proteins

Reactive Species

A05683 is reactive to RAB21 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

24348 MW

Background of RAB21

RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α-tail of endocytosed integrin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05683 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For RAB21 (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0118, Ras-related protein Rab-21 RAB21 RAB21, member RAS oncogene family ras-related protein Rab-21|GTP-binding protein RAB21

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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