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Product Info Summary
| SKU: | M00617 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-RANTES CCL5 Monoclonal Antibody
View all CCL5/RANTES Antibodies
SKU/Catalog Number
M00617
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RANTES CCL5 Monoclonal Antibody catalog # M00617. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RANTES CCL5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00617)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADHH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RANTES.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00617 is reactive to CCL5 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
250 kDa
Calculated molecular weight
49967 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00617 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of RANTES expression in RANTES recombinant protein lysate.
Protein Target Info & Infographic
Gene/Protein Information For CCL5 (Source: Uniprot.org, NCBI)
Gene Name
CCL5
Full Name
C-C motif chemokine 5
Weight
49967 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
Tyrosine-protein phosphatase non-receptor type 1;3.1.3.48;Protein-tyrosine phosphatase 1B;PTP-1B;PTPN1;PTP1B; CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCL5, check out the CCL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RANTES CCL5 Monoclonal Antibody (M00617)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-RANTES CCL5 Monoclonal Antibody
Question
We have observed staining in human leukocyte. Do you have any suggestions? Is anti-RANTES Monoclonal antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
From what I have seen in literature leukocyte does express CCL5. From what I have seen in Uniprot.org, CCL5 is expressed in blood, leukocyte, brain, among other tissues. Regarding which tissues have CCL5 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 16791620
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-23
Question
Is a blocking peptide available for product anti-RANTES Monoclonal antibody (M00617)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We do provide the blocking peptide for product anti-RANTES Monoclonal antibody (M00617). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-02
Question
Will anti-RANTES Monoclonal antibody M00617 work on goat IF with blood?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Our lab technicians have not tested anti-RANTES Monoclonal antibody M00617 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RANTES Monoclonal antibody M00617 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-29
Question
Would M00617 anti-RANTES Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
As indicated on the product datasheet, M00617 anti-RANTES Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-RANTES Monoclonal antibody M00617 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-RANTES Monoclonal antibody (M00617) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
Question
My team were happy with the WB result of your anti-RANTES Monoclonal antibody. However we have seen positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is CCL5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
According to literature, leukocyte does express CCL5. Generally CCL5 expresses in secreted. Regarding which tissues have CCL5 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 16791620
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-14
Question
I have a question about product M00617, anti-RANTES Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Mitchell
Verified customer
Asked: 2018-08-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00617 anti-RANTES Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-03
Question
I see that the anti-RANTES Monoclonal antibody M00617 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-11
Question
Would anti-RANTES Monoclonal antibody M00617 work for IF with blood?
S. Yang
Verified customer
Asked: 2018-01-29
Answer
According to the expression profile of blood, CCL5 is highly expressed in blood. So, it is likely that anti-RANTES Monoclonal antibody M00617 will work for IF with blood.
Boster Scientific Support
Answered: 2018-01-29
Question
My lab would like to test anti-RANTES Monoclonal antibody M00617 on human blood for research purposes, then I may be interested in using anti-RANTES Monoclonal antibody M00617 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
The products we sell, including anti-RANTES Monoclonal antibody M00617, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-RANTES Monoclonal antibody M00617. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-08
Question
We ordered your anti-RANTES Monoclonal antibody for IF on brain in the past. I am using human, and We intend to use the antibody for WB next. I am looking for examining brain as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
M. Wu
Verified customer
Asked: 2016-06-29
Answer
I viewed the website and datasheets of our anti-RANTES Monoclonal antibody and it appears that M00617 has been validated on human in both IF and WB. Thus M00617 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-06-29
Question
Is this M00617 anti-RANTES Monoclonal antibody reactive to the isotypes of CCL5?
K. Brown
Verified customer
Asked: 2015-06-05
Answer
The immunogen of M00617 anti-RANTES Monoclonal antibody is A synthesized peptide derived from human RANTES. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-05
Question
Do you have a BSA free version of anti-RANTES Monoclonal antibody M00617 available?
J. Li
Verified customer
Asked: 2015-03-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RANTES Monoclonal antibody M00617 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-03-20
Question
I was wanting to use your anti-RANTES Monoclonal antibody for IF for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
E. Li
Verified customer
Asked: 2014-10-03
Answer
You can see on the product datasheet, M00617 anti-RANTES Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-03
Question
See below the WB image, lot number and protocol we used for blood using anti-RANTES Monoclonal antibody M00617. Please let me know if you require anything else.
T. Walker
Verified customer
Asked: 2013-12-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-27
