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Product Info Summary
| SKU: | M01622 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Rad18 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01622
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rad18 Rabbit Monoclonal Antibody catalog # M01622. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad18 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01622)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FFC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rad18
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01622 is reactive to RAD18 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
56223 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01622 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
FC 1:20
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of RAD18 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RAD18 (Source: Uniprot.org, NCBI)
Gene Name
RAD18
Full Name
E3 ubiquitin-protein ligase RAD18
Weight
56223 MW
Superfamily
RAD18 family
Alternative Names
E3 ubiquitin-protein ligase RAD18;6.3.2.-;Postreplication repair protein RAD18;hHR18;hRAD18;RING finger protein 73;RAD18;RNF73; RAD18 RNF73 RAD18 E3 ubiquitin protein ligase E3 ubiquitin-protein ligase RAD18|RAD18 homolog|RAD18, S. cerevisiae, homolog|RING finger protein 73|RING-type E3 ubiquitin transferase RAD18|hHR18|hRAD18|postreplication repair protein RAD18|postreplication repair protein hRAD18p
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD18, check out the RAD18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rad18 Rabbit Monoclonal Antibody (M01622)
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No publications found for M01622
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Customer Q&As
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4 Customer Q&As for Anti-Rad18 Rabbit Monoclonal Antibody
Question
We are currently using anti-Rad18 Rabbit Monoclonal antibody M01622 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Rad18 Rabbit Monoclonal antibody (M01622) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
Is a blocking peptide available for product anti-Rad18 Rabbit Monoclonal antibody (M01622)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We do provide the blocking peptide for product anti-Rad18 Rabbit Monoclonal antibody (M01622). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-23
Question
Does anti-Rad18 Rabbit Monoclonal antibody M01622 work on dog WB with placenta?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Our lab technicians have not tested anti-Rad18 Rabbit Monoclonal antibody M01622 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Rad18 Rabbit Monoclonal antibody M01622 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-19
Question
Will M01622 anti-Rad18 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
It shows on the product datasheet, M01622 anti-Rad18 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-04
