Anti-Rad18 Rabbit Monoclonal Antibody

RAD18 antibody

Boster Bio Anti-Rad18 Rabbit Monoclonal Antibody catalog # M01622. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01622
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-Rad18 Rabbit Monoclonal Antibody

View all RAD18 Antibodies

SKU/Catalog Number

M01622

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Rad18 Rabbit Monoclonal Antibody catalog # M01622. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rad18 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01622)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FFC-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Rad18

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01622 is reactive to RAD18 in Human, Mouse, Rat

Applications

M01622 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

56223 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:10000-1:20000
FC 1:20

Validation Images & Assay Conditions

Gene/Protein Information For RAD18 (Source: Uniprot.org, NCBI)

Gene Name

RAD18

Full Name

E3 ubiquitin-protein ligase RAD18

Weight

56223 MW

Superfamily

RAD18 family

Alternative Names

EC 6.3.2.-; hHR18; hRAD18; postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD18 homolog (S. cerevisiae); RAD18, S. cerevisiae, homolog; RING finger protein 73; RNF73E3 ubiquitin-protein ligase RAD18 RAD18 RNF73 RAD18 E3 ubiquitin protein ligase E3 ubiquitin-protein ligase RAD18|RAD18 homolog|RAD18, S. cerevisiae, homolog|RING finger protein 73|RING-type E3 ubiquitin transferase RAD18|hHR18|hRAD18|postreplication repair protein RAD18|postreplication repair protein hRAD18p

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAD18, check out the RAD18 Infographic

RAD18 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAD18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Rad18 Rabbit Monoclonal Antibody

Question

We are currently using anti-Rad18 Rabbit Monoclonal antibody M01622 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

The anti-Rad18 Rabbit Monoclonal antibody (M01622) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-22

Question

Is a blocking peptide available for product anti-Rad18 Rabbit Monoclonal antibody (M01622)?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

We do provide the blocking peptide for product anti-Rad18 Rabbit Monoclonal antibody (M01622). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-23

Question

Does anti-Rad18 Rabbit Monoclonal antibody M01622 work on dog WB with placenta?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

Our lab technicians have not tested anti-Rad18 Rabbit Monoclonal antibody M01622 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Rad18 Rabbit Monoclonal antibody M01622 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-19

Question

Will M01622 anti-Rad18 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-04

Answer

It shows on the product datasheet, M01622 anti-Rad18 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-04

Order DetailsPrice
M01622

100μL

$370
M01622-30ul

30μL sample

$99
M01622-carrier-free-1ml

Carrier Free

$2590

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M01622
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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