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Product Info Summary
| SKU: | A13748-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PUDP Antibody Picoband®
SKU/Catalog Number
A13748-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PUDP Antibody Picoband® catalog # A13748-1. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PUDP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13748-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PUDP recombinant protein (Position: M1-E228). Human PUDP shares 71.2% amino acid (aa) sequence identity with mouse PUDP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13748-1 is reactive to PUDP in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.249kDa
Background of HDHD1A
This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13748-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell, human Caco-2 whole cell, human A431 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PUDP using anti-PUDP antibody (A13748-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUDP antigen affinity purified polyclonal antibody (Catalog # A13748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUDP at approximately 25 kDa. The expected band size for PUDP is at 25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PUDP (Source: Uniprot.org, NCBI)
Gene Name
PUDP
Full Name
Pseudouridine-5'-phosphatase
Weight
25.249kDa
Superfamily
HAD-like hydrolase superfamily
Alternative Names
DXF68S1E5'-PsiMPase; EC 3.1.3.n6; family with sequence similarity 16, member A, X-linked; GS1FAM16AX; haloacid dehalogenase-like hydrolase domain containing 1; haloacid dehalogenase-like hydrolase domain containing 1A; Haloacid dehalogenase-like hydrolase domain-containing protein 1; Haloacid dehalogenase-like hydrolase domain-containing protein 1A; HDHD1Apseudouridine-5'-monophosphatase; Protein GS1 PUDP DXF68S1E, FAM16AX, GS1, HDHD1, HDHD1A pseudouridine 5-phosphatase pseudouridine-5-phosphatase|5-PsiMPase|family with sequence similarity 16, member A, X-linked|haloacid dehalogenase-like hydrolase domain containing 1|haloacid dehalogenase-like hydrolase domain containing 1A|haloacid dehalogenase-like hydrolase domain-containing protein 1|haloacid dehalogenase-like hydrolase domain-containing protein 1A|pseudouridine-5-monophosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PUDP, check out the PUDP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PUDP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PUDP Antibody Picoband® (A13748-1)
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