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Product Info Summary
| SKU: | A18279-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PRSS44 Antibody Picoband®
SKU/Catalog Number
A18279-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRSS44 Antibody Picoband® catalog # A18279-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PRSS44 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18279-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRSS44 recombinant protein (Position: L22-K340).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A18279-1 is reactive to PRSS44 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
24145 MW
Background of Prss44
Predicted to enable serine-type endopeptidase activity. Predicted to be involved in germ cell development; proteolysis; and spermatogenesis. Located in cytoplasm. Orthologous to human PRSS44P (serine protease 44, pseudogene).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18279-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRSS44 using anti-PRSS44 antibody (A18279-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS44 antigen affinity purified polyclonal antibody (Catalog # A18279-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS44 at approximately 45 kDa. The expected band size for PRSS44 is at 41 kDa.
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Figure 2. IF analysis of PRSS44 using anti-PRSS44 antibody (A18279-1).
PRSS44 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRSS44 Antibody (A18279-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PRSS44 (Source: Uniprot.org, NCBI)
Gene Name
PRSS44
Full Name
Serine protease 44
Weight
24145 MW
Superfamily
peptidase S1 family
Alternative Names
Placenta-specific protein 9; PLAC9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRSS44, check out the PRSS44 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRSS44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRSS44 Antibody Picoband® (A18279-1)
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