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Product Info Summary
| SKU: | A14920-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PRSS35 Antibody Picoband®
SKU/Catalog Number
A14920-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRSS35 Antibody Picoband® catalog # A14920-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PRSS35 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14920-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRSS35 recombinant protein (Position: H30-D340).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A14920-1 is reactive to PRSS35 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
19908 MW
Background of PRSS35
Proteolytic degradation of extracellular matrix components has been suggested to play an essential role for the occurrence of ovulation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are not required in this process. PRSS35, which was upregulated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovulation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granulosa cells of preovulatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14920-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PC-3 whole cell, rat C6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRSS35 using anti-PRSS35 antibody (A14920-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS35 antigen affinity purified polyclonal antibody (Catalog # A14920-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS35 at approximately 53 kDa. The expected band size for PRSS35 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PRSS35 (Source: Uniprot.org, NCBI)
Gene Name
PRSS35
Full Name
Inactive serine protease 35
Weight
19908 MW
Superfamily
peptidase S1 family
Alternative Names
Transmembrane protein 240;TMEM240;C1orf70; PRSS35 C6orf158, dJ223E3.1 serine protease 35 inactive serine protease 35|protease, serine 35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRSS35, check out the PRSS35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRSS35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRSS35 Antibody Picoband® (A14920-1)
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