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Product Info Summary
| SKU: | A07411 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein orai-2 ORAI2 Antibody
SKU/Catalog Number
A07411
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein orai-2 ORAI2 Antibody (Catalog # A07411). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ORAI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein orai-2 ORAI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07411)
Host
Rabbit
Contents
ORAI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ORAI2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2. The immunogen is located within the last 50 amino acids of ORAI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3.
Reactive Species
A07411 is reactive to ORAI2 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
28570 MW
Background of ORAI2
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07411 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1
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Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 μg/mL.
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Immunofluorescence of ORAI2 in Jurkat cells with ORAI2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ORAI2 (Source: Uniprot.org, NCBI)
Gene Name
ORAI2
Full Name
Protein orai-2
Weight
28570 MW
Superfamily
Orai family
Alternative Names
CBCIP2, C7orf19, MEM142B, TMEM142B, CBCIP2, PP1729, Protein orai-2, CAP-binding protein complex-interacting protein 2 ORAI2 C7orf19, CBCIP2, MEM142B, TMEM142B ORAI calcium release-activated calcium modulator 2 protein orai-2|CAP-binding protein complex interacting protein 2|H_NH0514P08.8|putative protein ORAI2-2|transmembrane protein 142B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ORAI2, check out the ORAI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ORAI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein orai-2 ORAI2 Antibody (A07411)
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