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Product Info Summary
| SKU: | A07284 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein bicaudal D homolog 1 BICD1 Antibody
SKU/Catalog Number
A07284
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein bicaudal D homolog 1 BICD1 Antibody (Catalog # A07284). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BICD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein bicaudal D homolog 1 BICD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07284)
Host
Rabbit
Contents
BICD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BICD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BICD1. The immunogen is located within the last 50 amino acids of BICD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.
Reactive Species
A07284 is reactive to BICD1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
110750 MW
Background of BICD1
BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07284 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BICD1 antibody can be used for detection of BICD1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BICD1 in Daudi cell lysate with BICD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of BICD1 in Daudi cells with BICD1 antibody at 2.5 μg/mL.
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Immunofluorescence of BICD1 in Daudi cells with BICD1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BICD1 (Source: Uniprot.org, NCBI)
Gene Name
BICD1
Full Name
Protein bicaudal D homolog 1
Weight
110750 MW
Superfamily
BicD family
Alternative Names
BICD, Protein bicaudal D homolog 1, Bic-D 1 BICD1 BICD, bic-D 1 BICD cargo adaptor 1 protein bicaudal D homolog 1|bicaudal D homolog 1|cytoskeleton-like bicaudal D protein homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BICD1, check out the BICD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BICD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein bicaudal D homolog 1 BICD1 Antibody (A07284)
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