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Product Info Summary
| SKU: | PB9883 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-RAB7/RAB7A Antibody Picoband®
SKU/Catalog Number
PB9883
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB7/RAB7A Antibody Picoband® catalog # PB9883. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAB7/RAB7A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9883)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAB7, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9883 is reactive to RAB7A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23490 MW
Background of RAB7A
Ras-related protein Rab-7a is a protein that in humans is encoded by the RAB7A gene. RAB7A functions as a key regulator in endo-lysosomal trafficking, governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positions, and endosome-lysosome transport through different protein-protein interaction cascades. Furthermore, RAB7A is involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes through modulation of SOX10 and the oncogene MYC. Mutations in the lysosomal pathway result in tumor progression in melanoma cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9883 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human A375 whole cell, huamn A431 whole cell, human HL-60 whole cell, human U87 whole cell, human MCF-7 whole cellhuman THP-1 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
ICC/IF: U20S cell, MCF7 cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RAB7 using anti-RAB7 antibody (PB9883).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: huamn A431 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates.
Lane 7: human THP-1 whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat lung tissue lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7 antigen affinity purified polyclonal antibody (Catalog # PB9883) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7 at approximately 23 kDa. The expected band size for RAB7 is at 23 kDa.
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Figure 2. IF analysis of RAB7 using anti-RAB7 antibody (PB9883).
RAB7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RAB7 Antibody (PB9883) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A431 cells using anti-RAB7 antibody (PB9883).
Overlay histogram showing A431 cells stained with PB9883 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB7 Antibody (PB9883,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 4. IF analysis of RAB7 using anti-RAB7 antibody (PB9883).
RAB7 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RAB7 Antibody (PB9883) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For RAB7A (Source: Uniprot.org, NCBI)
Gene Name
RAB7A
Full Name
Ras-related protein Rab-7a
Weight
23490 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-7a;RAB7A;RAB7; RAB7A CMT2B, PRO2706, RAB7 RAB7A, member RAS oncogene family ras-related protein Rab-7a|RAB7, member RAS oncogene family|Ras-associated protein RAB7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB7A, check out the RAB7A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB7/RAB7A Antibody Picoband® (PB9883)
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Customer Q&As
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4 Customer Q&As for Anti-RAB7/RAB7A Antibody Picoband®
Question
My question regards using your anti-RAB7/RAB7A antibody for multi-organism intracellular transport studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
I appreciate your inquiry. This PB9883 anti-RAB7/RAB7A antibody is tested on hela whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-RAB7/RAB7A antibody PB9883 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
A. Li
Verified customer
Asked: 2016-10-11
Answer
The anti-RAB7/RAB7A antibody (PB9883) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-11
Question
We have been able to see staining in human cervix carcinoma. Any tips? Is anti-RAB7/RAB7A antibody supposed to stain cervix carcinoma positively?
T. Walker
Verified customer
Asked: 2015-12-04
Answer
Based on literature cervix carcinoma does express RAB7A. Based on Uniprot.org, RAB7A is expressed in frontal cortex, placenta, liver, brain, lung, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have RAB7A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 14702039, 15489334
Melanoma, Pubmed ID: 12643545, 17081065
Placenta, Pubmed ID: 8954989
Boster Scientific Support
Answered: 2015-12-04
Question
My team were well pleased with the WB result of your anti-RAB7/RAB7A antibody. However we have observed positive staining in frontal cortex phagosome membrane using this antibody. Is that expected? Could you tell me where is RAB7A supposed to be expressed?
M. Walker
Verified customer
Asked: 2013-10-15
Answer
From literature, frontal cortex does express RAB7A. Generally RAB7A expresses in cytoplasmic vesicle, phagosome membrane. Regarding which tissues have RAB7A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 14702039, 15489334
Melanoma, Pubmed ID: 12643545, 17081065
Placenta, Pubmed ID: 8954989
Boster Scientific Support
Answered: 2013-10-15
