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Product Info Summary
| SKU: | M01480-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Profilin-1 Antibody
View all Profilin 1 Antibodies
SKU/Catalog Number
M01480-1
Size
50 µl
Description
Boster Bio Anti-Profilin-1 Antibody (Catalog # M01480-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Profilin-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01480-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Profilin-1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-140 amino acids from the human region of human Profilin-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01480-1 is reactive to PFN1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
15054 Da
Background of Profilin 1
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01480-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-Profilin-1 Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HUVEC whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: 293 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing NIH/3T3 cells stained with M01480-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01480-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Overlay histogram showing Hela cells stained with M01480-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01480-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For PFN1 (Source: Uniprot.org, NCBI)
Gene Name
PFN1
Full Name
Profilin-1
Weight
15054 Da
Superfamily
profilin family
Alternative Names
Profilin-1, Epididymis tissue protein Li 184a, Profilin I, PFN1 PFN1 ALS18 profilin 1 profilin-1|epididymis tissue protein Li 184a|profilin I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFN1, check out the PFN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Profilin-1 Antibody (M01480-1)
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