Product Info Summary
SKU: | A05805 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-PPP2R2B Antibody
SKU/Catalog Number
A05805
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PPP2R2B Antibody (Catalog # A05805). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP2R2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05805)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PPP2R2B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05805 is reactive to PPP2R2B in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
51710 MW
Background of PPP2R2B
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity By similarity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance By similarity.
Mayer R.E., Biochemistry 30:3589-3597(1991) [PubMed: 1849734].
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].
Schmutz J., Nature 431:268-274(2004) [PubMed: 15372022].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05805 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, using PPP2R2B antibody A05805.
Protein Target Info & Infographic
Gene/Protein Information For PPP2R2B (Source: Uniprot.org, NCBI)
Gene Name
PPP2R2B
Full Name
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
Weight
51710 MW
Superfamily
phosphatase 2A regulatory subunit B family
Alternative Names
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform;PP2A subunit B isoform B55-beta;PP2A subunit B isoform PR55-beta;PP2A subunit B isoform R2-beta;PP2A subunit B isoform beta;PPP2R2B; PPP2R2B B55BETA, PP2AB55BETA, PP2ABBETA, PP2APR55B, PP2APR55BETA, PR2AB55BETA, PR2ABBETA, PR2APR55BETA, PR52B, PR55-BETA, PR55BETA, SCA12 protein phosphatase 2 regulatory subunit Bbeta serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform|protein phosphatase 2, regulatory subunit B, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2R2B, check out the PPP2R2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2R2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP2R2B Antibody (A05805)
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Customer Q&As
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3 Customer Q&As for Anti-PPP2R2B Antibody
Question
I was wanting to use your anti-PPP2R2B antibody for WB for mouse corpus callosum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse corpus callosum identification?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
As indicated on the product datasheet, A05805 anti-PPP2R2B antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse corpus callosum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-PPP2R2B antibody A05805 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
B. Bhatt
Verified customer
Asked: 2018-06-04
Answer
The anti-PPP2R2B antibody (A05805) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-04
Question
Do you have a BSA free version of anti-PPP2R2B antibody A05805 available?
B. Carter
Verified customer
Asked: 2013-05-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PPP2R2B antibody A05805 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-06