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Product Info Summary
| SKU: | A14507-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PPP1R37 Antibody Picoband®
SKU/Catalog Number
A14507-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPP1R37 Antibody Picoband® catalog # A14507-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PPP1R37 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14507-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PPP1R37 recombinant protein (Position: R66-R390). Human PPP1R37 shares 97.5% and 97.2% amino acid (aa) sequence identity with mouse and rat PPP1R37, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14507-1 is reactive to PPP1R37 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
77.574kDa
Background of LRRC68
PPP1R37 can inhibit phosphatase activity of protein phosphatase 1 (PP1) complexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14507-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell, human A431 whole cell, human MCF-7 whole cell, human SiHa whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PPP1R37 using anti-PPP1R37 antibody (A14507-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R37 antigen affinity purified polyclonal antibody (Catalog # A14507-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R37 at approximately 75 kDa. The expected band size for PPP1R37 is at 78 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PPP1R37 antibody (A14507-1).
Overlay histogram showing MCF-7 cells stained with A14507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R37 Antibody (A14507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R37 (Source: Uniprot.org, NCBI)
Gene Name
PPP1R37
Full Name
Protein phosphatase 1 regulatory subunit 37
Weight
77.574kDa
Superfamily
PPP1R37 family
Alternative Names
KIAA1986; protein phosphatase 1, regulatory subunit 37
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R37, check out the PPP1R37 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R37: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1R37 Antibody Picoband® (A14507-1)
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