Anti-POLDIP2 Antibody Picoband®

Product Name

Anti-POLDIP2 Antibody Picoband®

View all POLDIP2 Antibodies

SKU/Catalog Number

A09581-4

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-POLDIP2 Antibody Picoband® catalog # A09581-4. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-POLDIP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09581-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Standard Protein

E.coli-derived human POLDIP2 recombinant protein (Position: Q81-Q324). Human POLDIP2 shares 99.2% amino acid (aa) sequence identity with mouse POLDIP2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09581-4 is reactive to POLDIP2 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

36 kDa

Background of POLDIP2

Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans. This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit, as well as with proliferating cell nuclear antigen. The encoded protein maybe play a role in the ability of the replication fork to bypass DNA lesions. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09581-4 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Positive Control

WB: human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human K562 whole cell, rat testis tissue, rat kidney tissue, mouse testis tissue, mouse kidney tissue
ICC/IF: U2OS cell
FCM: U251 cell

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of POLDIP2 using anti-POLDIP2 antibody (A09581-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLDIP2 antigen affinity purified polyclonal antibody (Catalog # A09581-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLDIP2 at approximately 36 kDa. The expected band size for POLDIP2 is at 42 kDa.

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Figure 2. IF analysis of POLDIP2 using anti-POLDIP2 antibody (A09581-4).
POLDIP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLDIP2 Antibody (A09581-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U251 cells using anti-POLDIP2 antibody (A09581-4).
Overlay histogram showing U251 cells stained with A09581-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLDIP2 Antibody (A09581-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For POLDIP2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZP586F1524; p38; PDIP38DKFZp586F1524,38 kDa DNA polymerase delta interaction protein; POLD4; polymerase (DNA-directed), delta interacting protein 2; polymerase delta interacting protein 38; polymerase delta-interacting protein 2 POLDIP2 PDIP38, POLD4, p38 DNA polymerase delta interacting protein 2 polymerase delta-interacting protein 2|38 kDa DNA polymerase delta interaction protein|polymerase (DNA) delta interacting protein 2|polymerase (DNA-directed), delta interacting protein 2|polymerase delta interacting protein 38

For more info on POLDIP2, check out the POLDIP2 Infographic

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In this infographic, you will see the following information for POLDIP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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