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Product Info Summary
| SKU: | A09581-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-POLDIP2 Antibody Picoband®
SKU/Catalog Number
A09581-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-POLDIP2 Antibody Picoband® catalog # A09581-4. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-POLDIP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09581-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human POLDIP2 recombinant protein (Position: Q81-Q324). Human POLDIP2 shares 99.2% amino acid (aa) sequence identity with mouse POLDIP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09581-4 is reactive to POLDIP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
42.033kDa
Background of POLDIP2
Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans. This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit, as well as with proliferating cell nuclear antigen. The encoded protein maybe play a role in the ability of the replication fork to bypass DNA lesions. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09581-4 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human K562 whole cell, rat testis tissue, rat kidney tissue, mouse testis tissue, mouse kidney tissue
ICC/IF: U2OS cell
FCM: U251 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POLDIP2 using anti-POLDIP2 antibody (A09581-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLDIP2 antigen affinity purified polyclonal antibody (Catalog # A09581-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLDIP2 at approximately 36 kDa. The expected band size for POLDIP2 is at 42 kDa.
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Figure 2. IF analysis of POLDIP2 using anti-POLDIP2 antibody (A09581-4).
POLDIP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLDIP2 Antibody (A09581-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U251 cells using anti-POLDIP2 antibody (A09581-4).
Overlay histogram showing U251 cells stained with A09581-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLDIP2 Antibody (A09581-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For POLDIP2 (Source: Uniprot.org, NCBI)
Gene Name
POLDIP2
Full Name
Polymerase delta-interacting protein 2
Weight
42.033kDa
Alternative Names
DKFZP586F1524; p38; PDIP38DKFZp586F1524,38 kDa DNA polymerase delta interaction protein; POLD4; polymerase (DNA-directed), delta interacting protein 2; polymerase delta interacting protein 38; polymerase delta-interacting protein 2 POLDIP2 PDIP38, POLD4, p38 DNA polymerase delta interacting protein 2 polymerase delta-interacting protein 2|38 kDa DNA polymerase delta interaction protein|polymerase (DNA) delta interacting protein 2|polymerase (DNA-directed), delta interacting protein 2|polymerase delta interacting protein 38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLDIP2, check out the POLDIP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLDIP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLDIP2 Antibody Picoband® (A09581-4)
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