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Product Info Summary
| SKU: | A05111-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-POLD3 Antibody Picoband®
SKU/Catalog Number
A05111-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-POLD3 Antibody Picoband® catalog # A05111-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-POLD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05111-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human POLD3 recombinant protein (Position: D11-K296). Human POLD3 shares 87.8% amino acid (aa) sequence identity with mouse POLD3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05111-2 is reactive to POLD3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
51.4kDa
Background of POLD3
DNA polymerase delta subunit 3 is an enzyme that in humans is encoded by the POLD3 gene. This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05111-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POLD3 using anti-POLD3 antibody (A05111-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLD3 antigen affinity purified polyclonal antibody (Catalog # A05111-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLD3 at approximately 70 kDa. The expected band size for POLD3 is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For POLD3 (Source: Uniprot.org, NCBI)
Gene Name
POLD3
Full Name
DNA polymerase delta subunit 3
Weight
51.4kDa
Alternative Names
DNA polymerase delta subunit 3; DNA polymerase delta subunit p66; DNA polymerase delta, subunit 3; KIAA0039MGC119643; MGC119642; P66; P68; polymerase (DNA directed), delta 3; polymerase (DNA-directed), delta 3, accessory subunit POLD3 P66, P68, PPP1R128 DNA polymerase delta 3, accessory subunit DNA polymerase delta subunit 3|DNA polymerase delta subunit C|DNA polymerase delta subunit p66|DNA polymerase delta subunit p68|Pol delta C subunit (p66)|polymerase (DNA) delta 3, accessory subunit|polymerase (DNA-directed), delta 3, accessory subunit|protein phosphatase 1, regulatory subunit 128
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLD3, check out the POLD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLD3 Antibody Picoband® (A05111-2)
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