Anti-POLD3 Antibody Picoband™

Product Name

Anti-POLD3 Antibody Picoband™

View all POLD3 Antibodies

SKU/Catalog Number

A05111-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-POLD3 Antibody Picoband™ catalog # A05111-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-POLD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05111-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human POLD3 recombinant protein (Position: D11-K296). Human POLD3 shares 87.8% amino acid (aa) sequence identity with mouse POLD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05111-2 is reactive to POLD3 in Human, Mouse, Rat

Applications

A05111-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

Background of POLD3

DNA polymerase delta subunit 3 is an enzyme that in humans is encoded by the POLD3 gene. This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of POLD3 using anti-POLD3 antibody (A05111-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLD3 antigen affinity purified polyclonal antibody (Catalog # A05111-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLD3 at approximately 70 kDa. The expected band size for POLD3 is at 51 kDa.

Gene/Protein Information For POLD3 (Source: Uniprot.org, NCBI)

Alternative Names

DNA polymerase delta subunit 3; DNA polymerase delta subunit p66; DNA polymerase delta, subunit 3; KIAA0039MGC119643; MGC119642; P66; P68; polymerase (DNA directed), delta 3; polymerase (DNA-directed), delta 3, accessory subunit POLD3 P66, P68, PPP1R128 DNA polymerase delta 3, accessory subunit DNA polymerase delta subunit 3|DNA polymerase delta subunit C|DNA polymerase delta subunit p66|DNA polymerase delta subunit p68|Pol delta C subunit (p66)|polymerase (DNA) delta 3, accessory subunit|polymerase (DNA-directed), delta 3, accessory subunit|protein phosphatase 1, regulatory subunit 128

For more info on POLD3, check out the POLD3 Infographic

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In this infographic, you will see the following information for POLD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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