Product Info Summary
SKU: | A08114 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PIK3AP1 Antibody Picoband™
View all BCAP/PIK3AP1 Antibodies
SKU/Catalog Number
A08114
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PIK3AP1 Antibody Picoband™ catalog # A08114. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PIK3AP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08114)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human PIK3AP1 recombinant protein (Position: H78-R583). Human PIK3AP1 shares 83.8% amino acid (aa) sequence identity with mouse PIK3AP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08114 is reactive to Pik3ap1 in Human
Applications
A08114 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
52588 MW
Background of BCAP/PIK3AP1
Phosphoinositide 3-kinase adapter protein 1 is an enzyme that in humans is encoded by the PIK3AP1 gene. BCAP (B cell adaptor for phosphoinositide 3-kinase (PI3K)), also known as PI3KAP1, participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K. BCAP null mice have deficient immune function, with B cells that exhibit enhanced apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a08114 pik3ap1 primary antibodies wb testing 1 a08114 pik3ap1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a08114-pik3ap1-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of PIK3AP1 using anti-PIK3AP1 antibody (A08114).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3AP1 antigen affinity purified polyclonal antibody (Catalog # A08114) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3AP1 at approximately 90 kDa. The expected band size for PIK3AP1 is at 90,95,100 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Pik3ap1 (Source: Uniprot.org, NCBI)
Gene Name
Pik3ap1
Full Name
Phosphoinositide 3-kinase adapter protein 1
Weight
52588 MW
Alternative Names
1810044J04Rik; B cell adaptor protein; BCAP; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3 Pik3ap1|1810044J04Rik, BC, BCAP|phosphoinositide-3-kinase adaptor protein 1|phosphoinositide 3-kinase adapter protein 1|B cell phosphoinositide 3-kinase adaptor|B-cell adapter for phosphoinositide 3-kinase|B-cell phosphoinositide 3-kinase adapter protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pik3ap1, check out the Pik3ap1 Infographic
![Pik3ap1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pik3ap1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIK3AP1 Antibody Picoband™ (A08114)
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