Product Info Summary
SKU: | M00318 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-PI 3 Kinase p85 alpha PIK3R1 Rabbit Monoclonal Antibody
View all PI 3-Kinase p85 alpha Antibodies
SKU/Catalog Number
M00318
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PI 3 Kinase p85 alpha PIK3R1 Rabbit Monoclonal Antibody catalog # M00318. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase p85 alpha PIK3R1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00318)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CGB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PI 3 Kinase p85 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00318 is reactive to PIK3R1 in Human, Mouse, Rat
Applications
M00318 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
41 kDa
Calculated molecular weight
83598 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
![m00318 wb7 m00318 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m00318-wb7.jpg)
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
![m00318 wb10 m00318 wb10](https://www.bosterbio.com/media/catalog/product/m/0/m00318-wb10.jpg)
Click image to see more details
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
![m00318 wb8 m00318 wb8](https://www.bosterbio.com/media/catalog/product/m/0/m00318-wb8.jpg)
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
![m00318 wb m00318 wb](https://www.bosterbio.com/media/catalog/product/m/0/m00318-wb.jpg)
Click image to see more details
Western blot analysis of PI 3 Kinase p85 alpha expression in A431 cell lysate.
![m00318 wb9 m00318 wb9](https://www.bosterbio.com/media/catalog/product/m/0/m00318-wb9.jpg)
Click image to see more details
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For PIK3R1 (Source: Uniprot.org, NCBI)
Gene Name
PIK3R1
Full Name
Phosphatidylinositol 3-kinase regulatory subunit alpha
Weight
83598 MW
Superfamily
PI3K p85 subunit family
Alternative Names
GRB1; GRB1PI3-kinase subunit p85-alpha; p85; p85-ALPHA; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory, 1; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI 3Kinase p85 alpha; PI 3-Kinase p85 alpha; PI3K regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory s PIK3R1 AGM7, GRB1, IMD36, p85, p85-ALPHA phosphoinositide-3-kinase regulatory subunit 1 phosphatidylinositol 3-kinase regulatory subunit alpha|PI3-kinase subunit p85-alpha|PI3K regulatory subunit alpha|phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase regulatory subunit alpha|phosphoinositide-3-kinase, regulatory subunit 1 (alpha)|ptdIns-3-kinase regulatory subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIK3R1, check out the PIK3R1 Infographic
![PIK3R1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIK3R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PI 3 Kinase p85 alpha PIK3R1 Rabbit Monoclonal Antibody (M00318)
Hello CJ!
M00318 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model
Güleç İ, Şengelen A,Karagöz-Güzey F,Önay-Uçar E,Eren B,Vahabova G,Karacan M,Bilgen Özcan T.The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model.Brain Res.2021 Apr 27:147508.doi:10.1016/j.brainres.2021.147508.Epub ahead of print.PMID:33930376.
Species: Rat
M00318 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:1000
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