Anti-PHYHIPL Antibody Picoband™

Product Name

Anti-PHYHIPL Antibody Picoband™

View all Phyhipl Antibodies

SKU/Catalog Number

A14122-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PHYHIPL Antibody Picoband™ catalog # A14122-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PHYHIPL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14122-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human PHYHIPL recombinant protein (Position: M1-D336). Human PHYHIPL shares 97.3% and 96.7% amino acid (aa) sequence identity with mouse and rat PHYHIPL, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14122-1 is reactive to Phyhipl in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

Calculated molecular weight

Background of Phyhipl

Phytanoyl-CoA hydroxylase-interacting protein-like is an enzyme that in humans is encoded by the PHYHIPL gene. PHYHIPL (phytanoyl-CoA 2-hydroxylase interacting protein-like), also known as phytanoyl-CoA hydroxylase-interacting protein-like, is a 376 amino acid protein that contains one fibronectin type-III domain and belongs to the PHYHIP family. Conserved in chimpanzee, canine, mouse, rat, chicken, zebrafish and Caenorhabditis elegans, PHYHIPL exists as three alternatively spliced isoforms. PHYHIPL is a down-regulated target of IRX1, a homeobox tumor suppressor gene linked to gastric carcinoma. PHYHIPL may also play a role in the development of the central system. The gene that encodes PHYHIPL maps to human chromosome 10q21.1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14122-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PHYHIPL using anti-PHYHIPL antibody (A14122-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHYHIPL antigen affinity purified polyclonal antibody (Catalog # A14122-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHYHIPL at approximately 40 kDa. The expected band size for PHYHIPL is at 42,40,6 kDa.

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Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-PHYHIPL antibody (A14122-1).
Overlay histogram showing CACO-2 cells stained with A14122-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHYHIPL Antibody (A14122-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Phyhipl (Source: Uniprot.org, NCBI)

Alternative Names

Em:AC025038.1; KIAA1796phytanoyl-CoA hydroxylase interacting protein-like; phytanoyl-CoA 2-hydroxylase interacting protein-like; phytanoyl-CoA hydroxylase-interacting protein-like

For more info on Phyhipl, check out the Phyhipl Infographic

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In this infographic, you will see the following information for Phyhipl: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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